Efficient protein production requires selection at the vector: coding sequence junction
(English)Manuscript (preprint) (Other academic)
Here we show that the junction formed when a coding sequence is cloned into an expression vector can affect protein production levels by up to a 1000-fold. Optimised junctions can be identified after a post-cloning optimisation step, which generates a library of sequence variants that differ only in ribosome-binding sites. The approach is simple, inexpensive and applicable to any experiment where efficient expression of a cloned coding sequence is sought.
protein production, mrna structure, coding sequence, efficient expression
Biochemistry and Molecular Biology
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:su:diva-116602OAI: oai:DiVA.org:su-116602DiVA: diva2:807047