Vascular toxicity of ultra-small TiO2 nanoparticles and single walled carbon nanotubes in vitro and in vivo
(English)Manuscript (preprint) (Other academic)
Ultra-small nanoparticles (USNPs) at 1-3 nm are a subset of nanoparticles (NPs) that exhibit intermediate physicochemical properties between molecular dispersions and larger NPs. Despite interest in their utilization in applications such as theranostics, limited data about their toxicity exist. Here the effect of TiO2-USNPs on endothelial cells in vitro, and zebrafish embryos in vivo, was studied. The findings were compared to TiO2-NPs (30 nm) and single walled carbon nanotubes (SWCNTs). TiO2-USNPs were not cytotoxic, had no oxidative ability yet were genotoxic in vitro. They caused mortality at high concentrations in water possibly by acidifying the water and caused malformations in the form of pericardial edema when injected in early developing zebrafish embryos. Myo1C involved in glomerular development of zebrafish embryos was upregulated in embryos exposed to TiO2-USNPs. They also exhibited anti-angiogenic effects both in vitro and in vivo plus decreased nitric oxide concentration. TiO2-NPs were genotoxic but not cytotoxic. SWCNTs were cytotoxic in vitro and had the highest oxidative ability. Neither of these NPs had significant effects in vivo. To our knowledge this is the first study evaluating the effects of TiO2-USNPs on vascular toxicity in vitro and in vivo, demonstrating their potency and necessity for more focus in nanotoxicology.
Ultra small nanoparticles, Zebrafish. Endothelium, Angiogenesis, Genotoxicity
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:su:diva-117024OAI: oai:DiVA.org:su-117024DiVA: diva2:809744