Multicolor Fluorescent Labeling of Cellulose Nanofibrils by Click Chemistry
2015 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 16, no 4, 1293-1300 p.Article in journal (Refereed) Published
We have chemically modified cellulose nanofibrils (CNF) with furan and maleimide groups, and selectively labeled the modified CNF with fluorescent probes; 7-mercapto-4-methylcoumarin and fluorescein diacetate 5-maleimide, through two specific click chemistry reactions: Diels-Alder cycloaddition and the thiol-Michael reaction. Characterization by solid-state C-13 NMR and infrared spectroscopy was used to follow the surface modification and estimate the substitution degrees. We demonstrate that the two luminescent dyes could be selectively labeled onto CNF, yielding a multicolor CNF that was characterized by UV/visible and fluorescence spectroscopies. It was demonstrated that the multicolor CNF could be imaged using a confocal laser scanning microscope.
Place, publisher, year, edition, pages
2015. Vol. 16, no 4, 1293-1300 p.
Biological Sciences Chemical Sciences
IdentifiersURN: urn:nbn:se:su:diva-117490DOI: 10.1021/acs.biomac.5b00083ISI: 000353006000024PubMedID: 25774999OAI: oai:DiVA.org:su-117490DiVA: diva2:815548