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Multicolor Fluorescent Labeling of Cellulose Nanofibrils by Click Chemistry
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
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2015 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 16, no 4, 1293-1300 p.Article in journal (Refereed) Published
Abstract [en]

We have chemically modified cellulose nanofibrils (CNF) with furan and maleimide groups, and selectively labeled the modified CNF with fluorescent probes; 7-mercapto-4-methylcoumarin and fluorescein diacetate 5-maleimide, through two specific click chemistry reactions: Diels-Alder cycloaddition and the thiol-Michael reaction. Characterization by solid-state C-13 NMR and infrared spectroscopy was used to follow the surface modification and estimate the substitution degrees. We demonstrate that the two luminescent dyes could be selectively labeled onto CNF, yielding a multicolor CNF that was characterized by UV/visible and fluorescence spectroscopies. It was demonstrated that the multicolor CNF could be imaged using a confocal laser scanning microscope.

Place, publisher, year, edition, pages
2015. Vol. 16, no 4, 1293-1300 p.
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Biological Sciences Chemical Sciences
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URN: urn:nbn:se:su:diva-117490DOI: 10.1021/acs.biomac.5b00083ISI: 000353006000024PubMedID: 25774999OAI: oai:DiVA.org:su-117490DiVA: diva2:815548
Note

AuthorCount:7;

Available from: 2015-06-01 Created: 2015-05-20 Last updated: 2017-12-04Bibliographically approved

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Navarro, Julien R. G.Yu, YangFall, Andreas B.Mathew, RennyEden, MattiasBergström, Lennart
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