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Structures and gene clusters of the closely related O-antigens of Escherichia coli O46 and O134, both containing D-glucuronoyl-D-allothreonine
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Number of Authors: 9
2015 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 409, 20-24 p.Article in journal (Refereed) Published
Abstract [en]

The O-polysaccharides (O-antigens) were isolated by mild acid degradation of the lipopolysaccharide (LPS) of Escherichia coli O46 and O134. The structures of their linear tetrasaccharide repeating units were established by sugar analysis along with 1D and 2D H-1 and C-13 NMR spectroscopy: [GRAPHICS] where D-aThr indicates D-allothreonine and R indicates O-acetyl substitution (similar to 70% on aThr and similar to 15% on GalNAc) in E. coli O46 whereas the O-acetylation is absent in E. coli O134. Functions of genes in the essentially identical O-antigen gene clusters of E. coli O46 and O134 were tentatively assigned by a comparison with sequences in available databases and found to be in agreement with the O-polysaccharide structures established.

Place, publisher, year, edition, pages
2015. Vol. 409, 20-24 p.
Keyword [en]
Escherichia coli, O-polysaccharide, O-antigen, Bacterial polysaccharide structure, O-antigen gene cluster, Allothreonine
National Category
Biological Sciences Organic Chemistry
URN: urn:nbn:se:su:diva-118349DOI: 10.1016/j.carres.2015.03.014ISI: 000354570100003OAI: diva2:822743
Available from: 2015-06-17 Created: 2015-06-15 Last updated: 2015-06-17Bibliographically approved

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Widmalm, Göran
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Department of Organic Chemistry
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