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Three-phase molecularly imprinted sol-gel based hollow fiber liquid-phase microextraction combined with liquid chromatography-tandem mass spectrometry for enrichment and selective determination of a tentative lung cancer biomarker
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Number of Authors: 5
2015 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 995, 38-45 p.Article in journal (Refereed) Published
Abstract [en]

In the present study, the modification of a polysulfone hollow fiber membrane with in situ molecularly imprinted sol-gel process (as a novel and one-step method) was prepared and investigated. 3-(propylmethaaylate)trimethoxysilane (3PMTMOS) as an inorganic precursor was used for preparation of molecularly imprinted sol-gel. The modified molecularly imprinted sol-gel hollow fiber membrane (MSHM) was used for the liquid-phase microextraction (LPME) of hippuric acid (HA) in human plasma and urine samples. MSHM as a selective, robust, and durable tool was used for at least 50 extractions without significant decrease in the extraction efficiency. The non-molecularly imprinted sol-gel hollow fiber membrane (NSHM) as blank hollow fiber membrane was prepared by the same process, only without HA. To achieve the best condition, influential parameters on the extraction efficiency were thoroughly investigated. The capability of this robust, green, and simple method for extraction of HA was successfully accomplished with LC/MS/MS. The limits of detection (LOD) and quantification (LOQ) in human plasma and urine samples were 0.3 and 1.0 nmol L-1, respectively. The standard calibration curves were obtained within the concentration range 1-2000 nmol L-1 for HA in human plasma and urine. The coefficients of determination (r(2)) were >= 0.998. The obtained data exhibited recoveries were higher than 89% for the extraction of HA in human plasma and urine samples.

Place, publisher, year, edition, pages
2015. Vol. 995, 38-45 p.
Keyword [en]
Molecularly imprinted polymers, Sol-gel, Hollow fiber membrane, Hippuric acid, Liquid phase microextraction, Mass spectrometry
National Category
Biological Sciences Chemical Sciences
URN: urn:nbn:se:su:diva-119288DOI: 10.1016/j.jchromb.2015.05.005ISI: 000357243200006OAI: diva2:844318
Available from: 2015-08-05 Created: 2015-08-03 Last updated: 2015-08-05Bibliographically approved

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Abdel-Rehim, Mohamed
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Department of Environmental Science and Analytical Chemistry
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