Change search
ReferencesLink to record
Permanent link

Direct link
Unique proteomic signature for radiation sensitive patients; a comparative study between normo-sensitive and radiation sensitive breast cancer patients
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Show others and affiliations
Number of Authors: 9
2015 (English)In: Mutation research, ISSN 0027-5107, E-ISSN 1873-135X, Vol. 776, 128-135 p.Article in journal (Refereed) Published
Abstract [en]

Radiation therapy is a cornerstone of modern cancer treatment. Understanding the mechanisms behind normal tissue sensitivity is essential in order to minimize adverse side effects and yet to prevent local cancer reoccurrence. The aim of this study was to identify biomarkers of radiation sensitivity to enable personalized cancer treatment. To investigate the mechanisms behind radiation sensitivity a pilot study was made where eight radiation-sensitive and nine normo-sensitive patients were selected from a cohort of 2914 breast cancer patients, based on acute tissue reactions after radiation therapy. Whole blood was sampled and irradiated in vitro with 0, 1, or 150 mGy followed by 3 h incubation at 37 degrees C. The leukocytes of the two groups were isolated, pooled and protein expression profiles were investigated using isotope-coded protein labeling method (ICPL). First, leukocytes from the in vitro irradiated whole blood from normo-sensitive and extremely sensitive patients were compared to the non-irradiated controls. To validate this first study a second ICPL analysis comparing only the non-irradiated samples was conducted. Both approaches showed unique proteomic signatures separating the two groups at the basal level and after doses of 1 and 150 mGy. Pathway analyses of both proteomic approaches suggest that oxidative stress response, coagulation properties and acute phase response are hallmarks of radiation sensitivity supporting our previous study on oxidative stress response. This investigation provides unique characteristics of radiation sensitivity essential for individualized radiation therapy.

Place, publisher, year, edition, pages
2015. Vol. 776, 128-135 p.
Keyword [en]
Biomarker, Low-dose radiation, Proteomics, Radiosensitivity, Radiotherapy, Breast cancer
National Category
Cancer and Oncology Biochemistry and Molecular Biology Medical Genetics Pharmaceutical Sciences Microbiology
URN: urn:nbn:se:su:diva-119166DOI: 10.1016/j.mrfmmm.2014.12.002ISI: 000356735900014OAI: diva2:845294
Available from: 2015-08-11 Created: 2015-07-29 Last updated: 2015-08-11Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Skiöld, SaraHarms-Ringdahl, MatsHaghdoost, Siamak
By organisation
Department of Molecular Biosciences, The Wenner-Gren Institute
In the same journal
Mutation research
Cancer and OncologyBiochemistry and Molecular BiologyMedical GeneticsPharmaceutical SciencesMicrobiology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 38 hits
ReferencesLink to record
Permanent link

Direct link