Change search
ReferencesLink to record
Permanent link

Direct link
Compaction of rolling circle amplification products increases signal integrity and signal-to-noise ratio
Show others and affiliations
Number of Authors: 14
2015 (English)In: Scientific Reports, ISSN 2045-2322, Vol. 5, 12317Article in journal (Refereed) Published
Abstract [en]

Rolling circle amplification (RCA) for generation of distinct fluorescent signals in situ relies upon the self-collapsing properties of single-stranded DNA in commonly used RCA-based methods. By introducing a cross-hybridizing DNA oligonucleotide during rolling circle amplification, we demonstrate that the fluorophore-labeled RCA products (RCPs) become smaller. The reduced size of RCPs increases the local concentration of fluorophores and as a result, the signal intensity increases together with the signal-to-noise ratio. Furthermore, we have found that RCPs sometimes tend to disintegrate and may be recorded as several RCPs, a trait that is prevented with our cross-hybridizing DNA oligonucleotide. These effects generated by compaction of RCPs improve accuracy of visual as well as automated in situ analysis for RCA based methods, such as proximity ligation assays (PLA) and padlock probes.

Place, publisher, year, edition, pages
2015. Vol. 5, 12317
National Category
Biological Sciences Medical Biotechnology
URN: urn:nbn:se:su:diva-119541DOI: 10.1038/srep12317ISI: 000358358900001OAI: diva2:847455
Available from: 2015-08-20 Created: 2015-08-17 Last updated: 2015-08-20Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Qian, XiaoyanKrzywkowski, TomaszNilsson, Mats
By organisation
Department of Biochemistry and BiophysicsScience for Life Laboratory (SciLifeLab)
In the same journal
Scientific Reports
Biological SciencesMedical Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 56 hits
ReferencesLink to record
Permanent link

Direct link