Isolation by 454-sequencing and characterization of polymorphic microsatellite markers in the tetraploid perennial herb Campanula rotundifolia
Number of Authors: 4
2015 (English)In: Conservation Genetics Resources, ISSN 1877-7252, E-ISSN 1877-7260, Vol. 7, no 3, 721-722 p.Article in journal (Refereed) Published
Campanula rotundifolia is a perennial polyploid herb with a wide circumpolar distribution growing in a range of grassland, heathland and dune communities. The species is showing signs of strong decline in most parts of its range due to habitat degradation. Eight polymorphic microsatellites were developed using a 454-sequencing approach and subsequently characterized in 323 tetraploid individuals from 15 populations in a fragmented landscape in central Sweden. Between 7 and 26 alleles were observed per locus with observed and expected heterozygosity ranging between 0.71-0.98 and 0.71-0.93, respectively. C. rotundifolia may serve as a model species for studying the relative importance of life-history traits in genetic diversity responses to environmental changes.
Place, publisher, year, edition, pages
2015. Vol. 7, no 3, 721-722 p.
Environmental changes, Microsatellites, Polyploidy, Traits
IdentifiersURN: urn:nbn:se:su:diva-120901DOI: 10.1007/s12686-015-0443-5ISI: 000359521500027OAI: oai:DiVA.org:su-120901DiVA: diva2:856827