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Genetic determinants of anti-malarial acquired immunity in a large multi-centre study
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Number of Authors: 57
2015 (English)In: Malaria Journal, ISSN 1475-2875, Vol. 14, 333Article in journal (Refereed) Published
Abstract [en]

Background: Many studies report associations between human genetic factors and immunity to malaria but few have been reliably replicated. These studies are usually country-specific, use small sample sizes and are not directly comparable due to differences in methodologies. This study brings together samples and data collected from multiple sites across Africa and Asia to use standardized methods to look for consistent genetic effects on anti-malarial antibody levels. Methods: Sera, DNA samples and clinical data were collected from 13,299 individuals from ten sites in Senegal, Mali, Burkina Faso, Sudan, Kenya, Tanzania, and Sri Lanka using standardized methods. DNA was extracted and typed for 202 Single Nucleotide Polymorphisms with known associations to malaria or antibody production, and antibody levels to four clinical grade malarial antigens [AMA1, MSP1, MSP2, and (NANP) 4] plus total IgE were measured by ELISA techniques. Regression models were used to investigate the associations of clinical and genetic factors with antibody levels. Results: Malaria infection increased levels of antibodies to malaria antigens and, as expected, stable predictors of anti-malarial antibody levels included age, seasonality, location, and ethnicity. Correlations between antibodies to blood-stage antigens AMA1, MSP1 and MSP2 were higher between themselves than with antibodies to the (NANP)(4) epitope of the pre-erythrocytic circumsporozoite protein, while there was little or no correlation with total IgE levels. Individuals with sickle cell trait had significantly lower antibody levels to all blood-stage antigens, and recessive homozygotes for CD36 (rs321198) had significantly lower anti-malarial antibody levels to MSP2. Conclusion: Although the most significant finding with a consistent effect across sites was for sickle cell trait, its effect is likely to be via reducing a microscopically positive parasitaemia rather than directly on antibody levels. However, this study does demonstrate a framework for the feasibility of combining data from sites with heterogeneous malaria transmission levels across Africa and Asia with which to explore genetic effects on anti-malarial immunity.

Place, publisher, year, edition, pages
2015. Vol. 14, 333
Keyword [en]
Malaria, Antibody, Sickle cell trait, HbAS, CD36, Genotype
National Category
Immunology Microbiology in the medical area
URN: urn:nbn:se:su:diva-121516DOI: 10.1186/s12936-015-0833-xISI: 000360108800002OAI: diva2:860008
Available from: 2015-10-09 Created: 2015-10-05 Last updated: 2015-10-09Bibliographically approved

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Troye-Blomberg, Marita
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Department of Molecular Biosciences, The Wenner-Gren Institute
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