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Detecting a wide range of environmental contaminants in human blood samples-combining QuEChERS with LC-MS and GC-MS methods
Stockholm University, Faculty of Science, Department of Environmental Science and Analytical Chemistry. Helmholtz Centre for Environmental Research - UFZ, Germany.
Number of Authors: 4
2015 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 407, no 23, 7047-7054 p.Article in journal (Refereed) Published
Abstract [en]

Exposure to environmental pollution and consumer products may result in an uptake of chemicals into human tissues. Several studies have reported the presence of diverse environmental contaminants in human blood samples. However, previously developed multi-target methods for the analysis of human blood include a fairly limited amount of compounds stemming from one or two related compound groups. Thus, the sample preparation method QuEChERS (quick easy cheap effective rugged and safe) was tested for the extraction of 64 analytes covering a broad compound domain followed by detection using liquid and gas chromatography coupled to mass spectrometry (LC- and GC-MS). Forty-seven analytes showed absolute recoveries above 70 % in the first QuEChERS step, being a simple liquid-liquid extraction (LLE) using acetonitrile and salt. The second QuEChERS step, being a dispersive solid phase extraction, did not result in an overall improvement of recoveries or removal of background signals. Using solely the LLE step, eight analytes could subsequently be detected in human blood samples from the German Environmental Specimen Bank. Using a LC-multiple reaction monitoring (MRM) method with a triple quadrupole instrument, better recoveries were achieved than with an older LC-high-resolution (HR) MS full scan orbitrap instrument, which required a higher concentration factor of the extracts. However, the application of HRMS full scan methods could be used for the detection of additional compounds retrospectively.

Place, publisher, year, edition, pages
2015. Vol. 407, no 23, 7047-7054 p.
Keyword [en]
Multi-target screening, Environmental pollutants, Human blood, QuEChERS, LC-MS, GC-MS
National Category
Biological Sciences Chemical Sciences
Identifiers
URN: urn:nbn:se:su:diva-121497DOI: 10.1007/s00216-015-8857-1ISI: 000360412100014OAI: oai:DiVA.org:su-121497DiVA: diva2:860586
Available from: 2015-10-13 Created: 2015-10-05 Last updated: 2017-12-01Bibliographically approved

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