Identification and characterization of nuclear envelope protein interactions
2015 (English)Licentiate thesis, comprehensive summary (Other academic)
The Nuclear envelope which surrounds the chromatin of eukaryotic cells contains more than a hundred transmembrane proteins. Mutations in some genes encoding nuclear envelope proteins give rise to human diseases including neurological disorders. The function of many nuclear envelope proteins is not well established. This is partly because nuclear envelope proteins and their interactions are difficult to study due to the inherent resistance to extraction of nuclear envelope proteins. We have developed a novel method called MCLIP, to identify interacting partners of nuclear envelope proteins in live cells. Using MCLIP, we found three new binding partners of the inner nuclear membrane protein Samp1: the intermediate filament protein Lamin B1, the LINC complex protein Sun1 and the G-protein Ran. Furthermore, using in vitro studies, we show that Samp1 binds both Emerin and Ran directly. We have also studied the interaction between Samp1 and Ran in detail.
Place, publisher, year, edition, pages
Stockholm: Department of Neurochemistry, Stockholm University , 2015. , 48 p.
Samp1, MCLIP, Nuclear envelope, Ran, Emerin
Chemical Sciences Biological Sciences Cell and Molecular Biology
Research subject Neurochemistry with Molecular Neurobiology
IdentifiersURN: urn:nbn:se:su:diva-122052ISBN: 978-91-7649-289-5OAI: oai:DiVA.org:su-122052DiVA: diva2:862387
2015-11-04, Heilbronnsalen, C458, Svante Arrhenius väg 16 B, Stockholm, 13:00 (English)
Nilsson, Ing-Marie, FDMannervik, Bengt, Professor
Hallberg, Einar, Professor
FunderSwedish Research Council, 621-2010-448Swedish Cancer Society, 110590Stiftelsen Olle Engkvist Byggmästare
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