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Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Mathematics.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Number of Authors: 3
2015 (English)In: Journal of Cell Biology, ISSN 0021-9525, E-ISSN 1540-8140, Vol. 211, no 1, 63-75 p.Article in journal (Refereed) Published
Abstract [en]

Eukaryotic gene expression requires the ordered association of numerous factors with precursor messenger RNAs (premRNAs)/messenger RNAs (mRNAs) to achieve efficiency and regulation. Here, we use the Balbiani ring (BR) genes to demonstrate the temporal and spatial association of the exon junction complex (EJC) core with gene-specific endogenous premRNAs and mRNAs. The EJC core components bind cotranscriptionally to BR premRNAs during or very rapidly after splicing. The EJC core does not recruit the nonsense-mediated decay mediaters UPF2 and UPF3 until the BR messenger RNA protein complexes (mRNPs) enter the interchromatin. Even though several known adapters for the export factor NXF1 become part of BR mRNPs already at the gene, NXF1 binds to BR mRNPs only in the interchromatin. In steady state, a subset of the BR mRNPs in the interchromatin binds NXF1, UPF2, and UPF3. This binding appears to occur stochastically, and the efficiency approximately equals synthesis and export of the BR mRNPs. Our data provide unique in vivo information on how export competent eukaryotic mRNPs are formed.

Place, publisher, year, edition, pages
2015. Vol. 211, no 1, 63-75 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-122934DOI: 10.1083/jcb.201412017ISI: 000362729900009OAI: oai:DiVA.org:su-122934DiVA: diva2:871419
Available from: 2015-11-13 Created: 2015-11-11 Last updated: 2017-12-01Bibliographically approved

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Björk, PetraPersson, Jan-OlovWieslander, Lars
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CiteExportLink to record
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