Change search
ReferencesLink to record
Permanent link

Direct link
Membrane insertion and topology of the amino-terminal domain TMD0 of multidrug-resistance associated protein 6 (MRP6)
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Show others and affiliations
2015 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 589, no 24, 3921-3928 p.Article in journal (Refereed) Published
Abstract [en]

The function of the ATP-binding cassette transporter MRP6 is unknown but mutations in its gene cause pseudoxanthoma elasticum. We have investigated the membrane topology of the N-terminal transmembrane domain TMD0 of MRP6 and the membrane integration and orientation propensities of its transmembrane segments (TMs) by glycosylation mapping. Results demonstrate that TMD0 has five TMs, an Nout-Cin topology and that the less hydrophobic TMs have strong preference for their orientation in the membrane that affects the neighboring TMs. Two disease-causing mutations changing the number of positive charges in the loops of TMD0 did not affect the membrane insertion efficiencies of the adjacent TMs.

Place, publisher, year, edition, pages
2015. Vol. 589, no 24, 3921-3928 p.
Keyword [en]
ATP-binding cassette transporter, Multidrug-resistance associated protein 6, ABCC6, Transmembrane domain, Pseudoxanthoma elasticum, Membrane protein insertion
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-124228DOI: 10.1016/j.febslet.2015.10.030ISI: 000367232900019PubMedID: 26545497OAI: oai:DiVA.org:su-124228DiVA: diva2:882804
Available from: 2015-12-15 Created: 2015-12-15 Last updated: 2016-01-18Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Tellgren-Roth, ÅsaLara, PatriciaNilsson, IngMarie
By organisation
Department of Biochemistry and Biophysics
In the same journal
FEBS Letters
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 34 hits
ReferencesLink to record
Permanent link

Direct link