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CPP-Based Delivery System for In Vivo Gene Delivery
Stockholm University, Faculty of Science, Department of Neurochemistry. Tartu University, Estonia.
2015 (English)In: Cell-Penetrating Peptides: Methods and Protocols / [ed] Ülo Langel, Springer-Verlag New York, 2015, Vol. 1324, 339-347 p.Chapter in book (Refereed)
Abstract [en]

The method presents most important steps in estimating a CPP-mediated reporter gene delivery in a mouse model. The method is applicable for administrating noncovalent CPP/pDNA complexes via i.v. injection and for analysis of luciferase levels in tissue homogenates. This method could be extended to analyze the delivery of different nucleic acid cargos with other types of delivery vectors. First, a simple method is presented for assessing the stability of complexes in blood after i.v. administration, based on quantitation of a fluorescently labeled nucleic acid. Secondly, a protocol is presented for assessing and analyzing luciferase levels in mouse organs.

Place, publisher, year, edition, pages
Springer-Verlag New York, 2015. Vol. 1324, 339-347 p.
Series
, Methods in Molecular Biology, ISSN 1064-3745 ; 1324
National Category
Medical Biotechnology Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-124908DOI: 10.1007/978-1-4939-2806-4_22ISI: 000376037700023PubMedID: 26202280ISBN: 978-1-4939-2805-7ISBN: 978-1-4939-2806-4OAI: oai:DiVA.org:su-124908DiVA: diva2:891238
Available from: 2016-01-06 Created: 2016-01-06 Last updated: 2016-06-20Bibliographically approved

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