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PepFects and NickFects for the Intracellular Delivery of Nucleic Acids
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry. Tartu University, Estonia.
2015 (English)In: Cell-Penetrating Peptides: Methods and Protocols / [ed] Ülo Langel, New York: Springer, 2015, Vol. 1324, 303-315 p.Chapter in book (Refereed)
Abstract [en]

Nucleic acids can be utilized in gene therapy to restore, alter, or silence gene functions. In order to reveal the biological activity nucleic acids have to reach their intracellular targets by passing through the plasma membrane, which is impermeable for these large and negatively charged molecules. Cell-penetrating peptides (CPPs) condense nucleic acids into nanoparticles using non-covalent complexation strategy and mediate their delivery into the cell, whereas the physicochemical parameters of the nanoparticles determine the interactions with the membranes, uptake mechanism, and subsequent intracellular fate. The nanoparticles are mostly internalized by endocytosis that leads to the entrapment of them in endosomal vesicles. Therefore design of new CPPs that are applicable for non-covalent complex formation strategy and harness endosomolytic properties is highly vital. Here we demonstrate that PepFects and NickFects are efficient vectors for the intracellular delivery of various nucleic acids.This chapter describes how to form CPP/pDNA nanoparticles, evaluate stable nanoparticles formation, and assess gene delivery efficacy.

Place, publisher, year, edition, pages
New York: Springer, 2015. Vol. 1324, 303-315 p.
Series
, Methods in Molecular Biology, ISSN 1064-3745 ; 1324
Keyword [en]
Cell-penetrating peptide, Co-incubation, Nanoparticle formation, Oligonucleotide delivery, PepFect, NickFect
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:su:diva-124907DOI: 10.1007/978-1-4939-2806-4_19ISI: 000376037700020PubMedID: 26202277ISBN: 978-1-4939-2805-7ISBN: 978-1-4939-2806-4OAI: oai:DiVA.org:su-124907DiVA: diva2:891239
Available from: 2016-01-06 Created: 2016-01-06 Last updated: 2016-06-20Bibliographically approved

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Hällbrink, MattiasLangel, Ülo
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