Change search
ReferencesLink to record
Permanent link

Direct link
SCARA Involvement in the Uptake of Nanoparticles Formed by Cell-Penetrating Peptides
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry. Tartu University, Estonia.
2015 (English)In: Cell-Penetrating Peptides: Methods and Protocols / [ed] Ülo Langel, Springer-Verlag New York, 2015, Vol. 1324, 163-174 p.Chapter in book (Refereed)
Abstract [en]

The investigation of uptake mechanisms for cell-penetrating peptides (CPPs) is and has been an ongoing project for as long as the peptides have been known, a time period that now spans over two decades. The ultimate answer is yet to be revealed and the current understanding is that no "one" mechanism will ever be found. The reason for this is that the uptake mechanism seems to be dependent on a multitude of factors that include which CPP, what cells are used, whether or not there is cargo and what the cargo is. CPPs are capable of delivering a variety of bio-macromolecules that are by themselves unable to enter into cells. Our group has reported on many different peptides in recent years, many aimed at delivering various oligonucleotide-based cargoes. These peptides have utilized the inherent positive charge of the peptides and some rationally designed modifications to non-covalently complex oligonucleotides and bring them into cells. In this chapter, we present a brief overview of the current proposals for the uptake mechanisms of CPPs and describe methods for detecting and evaluating the role of scavenger receptor class A receptors in the uptake of non-covalent cell-penetrating peptide:oligonucleotide complexes.

Place, publisher, year, edition, pages
Springer-Verlag New York, 2015. Vol. 1324, 163-174 p.
Series
, Methods in Molecular Biology, ISSN 1064-3745 ; 1324
Keyword [en]
Cell-penetrating peptides, CPPs, Splice-correction, Scavenger receptors, siRNA, Transfection, SCARA3, SCARA5, Uptake mechanism
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-124904DOI: 10.1007/978-1-4939-2806-4_11ISI: 000376037700012PubMedID: 26202269ISBN: 978-1-4939-2805-7ISBN: 978-1-4939-2806-4OAI: oai:DiVA.org:su-124904DiVA: diva2:891241
Available from: 2016-01-06 Created: 2016-01-06 Last updated: 2016-06-20Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Helmfors, HenrikLindberg, StaffanLangel, Ülo
By organisation
Department of Neurochemistry
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 20 hits
ReferencesLink to record
Permanent link

Direct link