Change search
ReferencesLink to record
Permanent link

Direct link
Structure elucidation and biosynthesis gene cluster organization of the O-antigen of Escherichia coli O170
Show others and affiliations
Number of Authors: 7
2015 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 417, 11-14 p.Article in journal (Refereed) Published
Abstract [en]

Enterotoxigenic Escherichia coli are causative agents of diarrhea in humans as well as animals, and E. coli O170 belongs to this virotype. Upon mild acid degradation of the lipopolysaccharide of E. coli O170, the branched O-polysaccharide chain was partially cleaved at beta-D-glactofuranosidic linkages to give multiple products, including a linear tetrasaccharide and oligomers thereof. Studies of the acid degradation products and O-deacylated lipopolysaccharide by 1D and 2D H-1 and C-13 NMR spectroscopy enabled elucidation of the following O-polysaccharide structure: -> 4)-beta-D-GlcpNAc-(1 -> 4)-beta-D-GlcpA-(1 -> 3)-beta-D-Galf-(1 -> 3)-beta-D-GlcNAc-(1 -> [GRAPHICS] beta-D-Galf Functions of genes in the O-antigen biosynthesis gene cluster were tentatively assigned and found to be in agreement with the O-polysaccharide structure.

Place, publisher, year, edition, pages
2015. Vol. 417, 11-14 p.
Keyword [en]
Escherichia coli, Lipopolysaccharide, O-polysaccharide, Bacterial polysaccharide structure, O-antigen gene cluster
National Category
Biological Sciences Chemical Sciences
URN: urn:nbn:se:su:diva-124748DOI: 10.1016/j.carres.2015.08.013ISI: 000364859900002PubMedID: 26382081OAI: diva2:892199
Available from: 2016-01-08 Created: 2016-01-04 Last updated: 2016-01-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Widmalm, Göran
By organisation
Department of Organic Chemistry
In the same journal
Carbohydrate Research
Biological SciencesChemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 14 hits
ReferencesLink to record
Permanent link

Direct link