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Structure elucidation and biosynthesis gene cluster organization of the O-antigen of Escherichia coli O170
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2015 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 417, p. 11-14Article in journal (Refereed) Published
Abstract [en]

Enterotoxigenic Escherichia coli are causative agents of diarrhea in humans as well as animals, and E. coli O170 belongs to this virotype. Upon mild acid degradation of the lipopolysaccharide of E. coli O170, the branched O-polysaccharide chain was partially cleaved at beta-D-glactofuranosidic linkages to give multiple products, including a linear tetrasaccharide and oligomers thereof. Studies of the acid degradation products and O-deacylated lipopolysaccharide by 1D and 2D H-1 and C-13 NMR spectroscopy enabled elucidation of the following O-polysaccharide structure: -> 4)-beta-D-GlcpNAc-(1 -> 4)-beta-D-GlcpA-(1 -> 3)-beta-D-Galf-(1 -> 3)-beta-D-GlcNAc-(1 -> [GRAPHICS] beta-D-Galf Functions of genes in the O-antigen biosynthesis gene cluster were tentatively assigned and found to be in agreement with the O-polysaccharide structure.

Place, publisher, year, edition, pages
2015. Vol. 417, p. 11-14
Keywords [en]
Escherichia coli, Lipopolysaccharide, O-polysaccharide, Bacterial polysaccharide structure, O-antigen gene cluster
National Category
Biological Sciences Chemical Sciences
Identifiers
URN: urn:nbn:se:su:diva-124748DOI: 10.1016/j.carres.2015.08.013ISI: 000364859900002PubMedID: 26382081OAI: oai:DiVA.org:su-124748DiVA, id: diva2:892199
Funder
Swedish Research CouncilAvailable from: 2016-01-08 Created: 2016-01-04 Last updated: 2018-03-20Bibliographically approved

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Widmalm, Göran
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