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A Flowchart to Analyze Protease Activity in Plant Mitochondria
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Number of Authors: 4
2015 (English)In: Plant Mitochondria: Methods and Protocols / [ed] James Whelan, Monika W. Murcha, Springer-Verlag New York, 2015, Vol. 1305, 123-130 p.Chapter in book (Refereed)
Abstract [en]

Proteases are one of the most abundant classes of enzymes and are involved in a plethora of biological processes in many cellular compartments, including the mitochondria. To understand the role of proteases is essential to determine their substrate repertoire, preferably in an in vivo setting. In this chapter we describe general guidelines to analyze protease activity using several strategies, from in-gel analysis to mass spectrometry mapping of the cleavage site(s) and fluorogenic probes that can easily be used in vivo. To exemplify this flowchart, we used the recently characterized organellar oligopeptidase of Arabidopsis (Arabidopsis thaliana), an enzyme that takes part in degradation of short peptides within mitochondria and chloroplasts.

Place, publisher, year, edition, pages
Springer-Verlag New York, 2015. Vol. 1305, 123-130 p.
Series
, Methods in Molecular Biology, ISSN 1064-3745 ; 1305
Keyword [en]
Peptide, Protease, Mitochondria, Electrophoresis, Mass spectrometry, Fluorogenic probe
National Category
Biochemistry and Molecular Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:su:diva-125889DOI: 10.1007/978-1-4939-2639-8_8ISI: 000366505400010PubMedID: 25910730ISBN: 978-1-4939-2639-8ISBN: 978-1-4939-2638-1OAI: oai:DiVA.org:su-125889DiVA: diva2:896176
Available from: 2016-01-20 Created: 2016-01-19 Last updated: 2016-01-20Bibliographically approved

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Teixeira, Pedro F.Kmiec, BeataGlaser, Elzbieta
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