Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Biomarker-enhanced assessment of reproductive disorders in Monoporeia affinis exposed to contaminated sediment in the Baltic Sea
Stockholm University, Faculty of Science, Department of Environmental Science and Analytical Chemistry.ORCID iD: 0000-0001-9522-5824
Stockholm University, Faculty of Science, Department of Environmental Science and Analytical Chemistry.
Stockholm University, Faculty of Science, Department of Environmental Science and Analytical Chemistry.
Stockholm University, Faculty of Science, Department of Environmental Science and Analytical Chemistry.
Show others and affiliations
2016 (English)In: Ecological Indicators, ISSN 1470-160X, E-ISSN 1872-7034, Vol. 63, p. 187-195Article in journal (Refereed) Published
Abstract [en]

Introducing biomarkers into monitoring programs requires understanding of their responses in relation to higher-level biological effects as well as modulating effects of confounding environmental factors. We evaluated relationships between the general toxicity biomarkers (acetylcholinesterase [AChE], lysosomal membrane stability [LMS], oxygen radical absorbance capacity [ORAC]) and reproductive performance (fecundity and embryo aberrations) in the amphipod Monoporeia affinis in the Baltic Sea. To further link biomarker response to contaminant (PCBs, PAHs and metals) levels in the surrounding sediments as well as environmental factors (salinity, bottom depth and total organic carbon in sediments [TOC]), correlation and partial least square regression (PLSR) analyses were applied. The observed contaminants levels were frequently elevated for heavy metals and PAHs, but not PCBs. In the amphipod populations, female ORAC values were positively related to the occurrence of females carrying malformed or membrane-damaged embryos and to the percentage of such embryos in their broods, but also to the fecundity. Female AChE activity was negatively related to the frequency of the membrane-damaged embryos, and positively to the frequency of embryos with arrested development in the broods. Moreover, higher AChE activity and ORAC values in the females occurred at elevated concentrations of metals and PAHs, while there was a negative correlation between embryo ORAC and some PCB congeners. The PLSR models explained over 80% of the variation in the female ORAC and AChE values by variation in contaminant concentrations in combination with environmental variables. Specifically, CB180 and PAM4,9 were identified as negative predictors for ORAC, whereas many PAHs and some metals were positive predictors. The AChE activity was positively related to some metals and negatively to PCBs. In the PLSR models, environmental factors had significant modulating effects, with positive effect of salinity on female ORAC and AChE, and negative effect of TOC on the AChE. The LMS data were less informative, with no apparent relation to any of the contaminants. Linking subcellular responses to the reproduction effects facilitates environmental stress assessment and understanding of the response mechanisms, but also calls for more experimental and field data providing a mechanistic understanding to these linkages.

Place, publisher, year, edition, pages
2016. Vol. 63, p. 187-195
Keywords [en]
Biomarkers of biological effects of contaminants in sediment, Acetylcholinesterase activity, Antioxidant status, Lysosomal membrane stability, Embryo aberrations, Amphipod Monoporeia affinis, Baltic Sea
National Category
Environmental Sciences
Research subject
Applied Environmental Science
Identifiers
URN: urn:nbn:se:su:diva-126021DOI: 10.1016/j.ecolind.2015.11.024ISI: 000370906000020OAI: oai:DiVA.org:su-126021DiVA, id: diva2:896756
Projects
BONUS + BEAST project
Funder
EU, FP7, Seventh Framework Programme, 217246Swedish Environmental Protection AgencyAvailable from: 2016-01-22 Created: 2016-01-22 Last updated: 2018-09-15Bibliographically approved
In thesis
1. Biomarkers and bioindicators of hypoxia and sediment toxicity in Monoporeia affinis
Open this publication in new window or tab >>Biomarkers and bioindicators of hypoxia and sediment toxicity in Monoporeia affinis
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Two of the most pressing environmental problems in the Baltic Sea are pollution and oxygen deficiency. The aim of this doctoral thesis was to evaluate a set of biomarkers and reproductive variables in the amphipod Monoporeia affinis to test their suitability as indicators of exposure to contaminants and hypoxia. In Paper I and Paper II, field-sampled M. affinis were exposed to varying oxygen conditions in combination with contaminated or reference sediment. Paper I showed that the combined effect of hypoxia and contaminants induced larger increases in antioxidant enzyme activities and higher levels of damaged lipids, resulting from oxidative stress, than single-stress treatments. The biomarkers AChE and GST responded only to the contaminant exposure. Paper II demonstrated that M. affinis can prepare against oxidative stress that facilitates recovery after a hypoxic episode, but that exposure to contaminants altered this response and resulted in higher baselines of antioxidants during hypoxia and no response upon reoxygenation. Paper III linked the occurrence of different embryo aberrations in M. affinis to concentrations of PAHs, PCBs and metals in sediments in the field. A key finding is that different types of embryo aberrations were associated with specific contaminant groups in the sediment. Paper IV addressed the link between biomarkers and bioindicators in M. affinis and found positive associations between increased antioxidant capacity in females and higher occurrence and frequencies of malformed and membrane-damaged embryos. AChE inhibition was linked to frequency of membrane-damaged embryos. Links between biomarker response and fecundity and embryo developmental stage were also established. Further, a large part of the variation in female antioxidant capacity and AChE activity could be explained by variation in concentrations of different metals, PAHs and PCB congeners, in combination with the environmental variables salinity, depth and sediment TOC.

Place, publisher, year, edition, pages
Stockholm: Department of Applied Environmental Science (ITM), Stockholm University, 2014. p. 35
Keywords
Biomarkers, Bioindicators, Embryo aberrations, Hypoxia, Contaminated sediment, PAHs, PCBs, Metals, Baltic Sea, Amphipods, Monoporeia affinis
National Category
Environmental Sciences
Research subject
Applied Environmental Science
Identifiers
urn:nbn:se:su:diva-102828 (URN)978-91-7447-905-8 (ISBN)
Public defence
2014-05-28, De Geersalen, Geovetenskapens hus, Svante Arrhenius väg 14, Stockholm, 10:00 (Swedish)
Opponent
Supervisors
Note

At the time of the doctoral defence the following papers were unpublished and had a status as follows: Paper 3: Manuscript; Paper 4: Manuscript.

Available from: 2014-05-06 Created: 2014-04-22 Last updated: 2018-09-15Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full text

Search in DiVA

By author/editor
Löf, MarieSundelin, BritaLiewenborg, BirgittaBandh, CeciliaGorokhova, Elena
By organisation
Department of Environmental Science and Analytical Chemistry
In the same journal
Ecological Indicators
Environmental Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 39 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf