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A fusion protein derived from Moraxella catarrhalis and Neisseria meningitidis aimed for immune modulation of human B cells
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
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Number of Authors: 6
2015 (English)In: Human Vaccines & Immunotherapeutics, ISSN 2164-5515, E-ISSN 2164-554X, Vol. 11, no 9, 2223-2227 p.Article in journal (Refereed) Published
Abstract [en]

Moraxella IgD-binding protein (MID) is a well characterized trimeric autotransporter that specifically targets the IgD of B cells. We fused the membrane anchor of the meningococcal autotransporter NhhA with the IgD-binding region of MID (aa 962-1200) to create a chimeric protein designated as NID. The aim was to use this specific targeting to provide a better vaccine candidate against meningococci, in particular serogroup B by enhancing the immunogenicity of NhhA. NID was thereafter recombinantly expressed in E. coli. The NID-expressing E. coli bound to peripheral B lymphocytes that resulted in cellular activation. Furthermore, we also successfully expressed NID on outer membrane vesicles, nanoparticles that are commonly used in meningococcal vaccines. This study thus highlights the applicability of the menigococcal-Moraxella fusion protein NID to be used for specific targeting of vaccine components to the IgD B cell receptor.

Place, publisher, year, edition, pages
2015. Vol. 11, no 9, 2223-2227 p.
Keyword [en]
B cells, MID, Neisseria meningitidis, NhhA, OMV, vaccine
National Category
Environmental Biotechnology Immunology in the medical area
Identifiers
URN: urn:nbn:se:su:diva-121661DOI: 10.1080/21645515.2015.1034917ISI: 000360493900024OAI: oai:DiVA.org:su-121661DiVA: diva2:900358
Available from: 2016-02-04 Created: 2015-10-13 Last updated: 2016-02-04Bibliographically approved

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Jonsson, Ann-Beth
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Department of Molecular Biosciences, The Wenner-Gren Institute
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Environmental BiotechnologyImmunology in the medical area

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