Change search
ReferencesLink to record
Permanent link

Direct link
The impact of salinity on the symbiosis between Casuarina glauca Sieb. ex Spreng. and N-2-fixing Frankia bacteria based on the analysis of Nitrogen and Carbon metabolism
Show others and affiliations
Number of Authors: 10
2016 (English)In: Plant and Soil, ISSN 0032-079X, E-ISSN 1573-5036, Vol. 398, no 1-2, 327-337 p.Article in journal (Refereed) Published
Abstract [en]

Casuarina glauca is an actinorhizal plant that establishes root-nodule symbiosis with N-2-fixing bacteria of the genus Frankia. This plant is highly recalcitrant to extreme environmental conditions such as salinity and drought. The aim of this study was to evaluate the impact of salt stress on the symbiotic relationship between C. glauca and Frankia Thr, focusing on N and C metabolism. Symbiotic and non-symbiotic plants were exposed to 0, 200, 400 and 600 mM NaCl. The following analyses were performed: stable carbon (delta C-13) and nitrogen (delta N-15) isotope signature; nitrogenase activity in nodules (acetylene reduction assay); and gene expression of a set of genes involved in nodule infection and N/C metabolism (qRT-PCR). Data were analysed using two-way ANOVA. Salt stress induced an enrichment in delta C-13 and delta N-15, reflecting a negative impact of salt in the relative water content and N-2 fixation, respectively. Furthermore, nitrogenase activity in nodules was insignificant already at 200 mM NaCl, consistent with the expression patterns of nifH as well as of plant genes involved in nodule induction and metabolism. The ability of C. glauca to thrive under highly saline environments is not dependent on the symbiosis with Frankia.

Place, publisher, year, edition, pages
2016. Vol. 398, no 1-2, 327-337 p.
Keyword [en]
Casuarina glauca, Frankia, Actinorhizal nodules, Salt stress
National Category
Agricultural Science, Forestry and Fisheries Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-128004DOI: 10.1007/s11104-015-2666-3ISI: 000368550700025OAI: oai:DiVA.org:su-128004DiVA: diva2:913298
Available from: 2016-03-21 Created: 2016-03-15 Last updated: 2016-03-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Pawlowski, Katharina
By organisation
Department of Ecology, Environment and Plant Sciences
In the same journal
Plant and Soil
Agricultural Science, Forestry and FisheriesBiological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 8 hits
ReferencesLink to record
Permanent link

Direct link