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Crystal structures reveal the molecular basis of ion translocation in sodium/proton antiporters
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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Number of Authors: 9
2016 (English)In: Nature Structural & Molecular Biology, ISSN 1545-9993, E-ISSN 1545-9985, Vol. 23, no 3, 248-255 p.Article in journal (Refereed) Published
Abstract [en]

To fully understand the transport mechanism of Na+/H+ exchangers, it is necessary to clearly establish the global rearrangements required to facilitate ion translocation. Currently, two different transport models have been proposed. Some reports have suggested that structural isomerization is achieved through large elevator-like rearrangements similar to those seen in the structurally unrelated sodium-coupled glutamate-transporter homolog Glt(ph). Others have proposed that only small domain movements are required for ion exchange, and a conventional rocking-bundle model has been proposed instead. Here, to resolve these differences, we report atomic-resolution structures of the same Na+/H+ antiporter (NapA from Thermus thermophilus) in both outward- and inward-facing conformations. These data combined with cross-linking, molecular dynamics simulations and isothermal calorimetry suggest that Na+/H+ antiporters provide alternating access to the ion-binding site by using elevator-like structural transitions.

Place, publisher, year, edition, pages
2016. Vol. 23, no 3, 248-255 p.
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Biological Sciences
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URN: urn:nbn:se:su:diva-128517DOI: 10.1038/nsmb.3164ISI: 000371452500012PubMedID: 26828964OAI: oai:DiVA.org:su-128517DiVA: diva2:917355
Available from: 2016-04-06 Created: 2016-03-30 Last updated: 2016-04-06Bibliographically approved

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Coincon, MathieuUzdavinys, PovilasNji, EmmanuelWinkelmann, IvenAbdul-Hussein, SabaDrew, David
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