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Transient delay of radiation-induced apoptosis by phorbol acetate
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Number of Authors: 4
2016 (English)In: Radiation and Environmental Biophysics, ISSN 0301-634X, E-ISSN 1432-2099, Vol. 55, no 1, 95-102 p.Article in journal (Refereed) Published
Abstract [en]

The mechanisms of interference of a model tumour promoter 12-O-tetra-decanoylphorbol-13-acetate (TPA) with radiation-induced apoptosis in human peripheral lymphocytes have been investigated. The cells were treated with TPA under various conditions and thereafter exposed to a single lethal dose of gamma radiation. Morphological and biochemical changes characteristic of apoptosis were followed up to 72 h of post-irradiation time. Acute exposure to low concentration of TPA resulted in delay in the onset of radiation-induced apoptosis (determined as morphological changes and rate of mitochondrial demise) by 24-48 h as compared to the irradiated, sham TPA-treated cells. The time course of this delay correlated well with confinement of the p53 protein to the cytoplasm and increase in bcl-2 levels at the nuclear periphery of irradiated cells. Our results indicate that confinement of p53 in the cytoplasm is one of the potential mechanisms by which TPA interferes with the process of radiation-induced apoptosis in human lymphocytes.

Place, publisher, year, edition, pages
2016. Vol. 55, no 1, 95-102 p.
Keyword [en]
Human lymphocytes, Radiation, 12-O-tetra-decanoylphorbol-13-acetate, Apoptosis, p53, bcl-2, Mitochondria
National Category
Biological Sciences Earth and Related Environmental Sciences Radiology, Nuclear Medicine and Medical Imaging
Identifiers
URN: urn:nbn:se:su:diva-128519DOI: 10.1007/s00411-015-0626-1ISI: 000371312400013PubMedID: 26581877OAI: oai:DiVA.org:su-128519DiVA: diva2:917358
Available from: 2016-04-06 Created: 2016-03-30 Last updated: 2016-04-06Bibliographically approved

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Olsson, GunillaCzene, StefanHaghdoost, SiamakHarms-Ringdahl, Mats
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Department of Molecular Biosciences, The Wenner-Gren Institute
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Radiation and Environmental Biophysics
Biological SciencesEarth and Related Environmental SciencesRadiology, Nuclear Medicine and Medical Imaging

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ReferencesLink to record
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