Change search
ReferencesLink to record
Permanent link

Direct link
Essential role of UCP1 modulating the central effects of thyroid hormones on energy balance
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Show others and affiliations
Number of Authors: 7
2016 (English)In: Molecular metabolism, ISSN 2212-8778, Vol. 5, no 4, 271-282 p.Article in journal (Refereed) Published
Abstract [en]

Objective: Classically, metabolic effects of thyroid hormones (THs) have been considered to be peripherally mediated, i.e. different tissues in the body respond directly to thyroid hormones with an increased metabolism. An alternative view is that the metabolic effects are centrally regulated. We have examined here the degree to which prolonged, centrally infused triiodothyronine (T3) could in itself induce total body metabolic effects and the degree to which brown adipose tissue (BAT) thermogenesis was essential for such effects, by examining uncoupling protein 1 (UCP1) KO mice. Methods: Wildtype and UPC1 KO mice were centrally-treated with T3 by using minipumps. Metabolic measurements were analyzed by indirect calorimetry and expression analysis by RT-PCR or western blot. BAT morphology and histology were studied by immunohistochemistry. Results: We found that central T3-treatment led to reduced levels of hypothalamic AMP-activated protein kinase (AMPK) and elevated body temperature (0.7 degrees C). UCP1 was essential for the T3-induced increased rate of energy expenditure, which was only observable at thermoneutrality and notably only during the active phase, for the increased body weight loss, for the increased hypothalamic levels of neuropeptide Y (NPY) and agouti-related peptide (AgRP) and for the increased food intake induced by central T3-treatment. Prolonged central T3-treatment also led to recruitment of BAT and britening/beiging (browning) of inguinal white adipose tissue (iWAT). Conclusions: We conclude that UCP1 is essential for mediation of the central effects of thyroid hormones on energy balance, and we suggest that similar UCP1-dependent effects may underlie central energy balance effects of other agents.

Place, publisher, year, edition, pages
2016. Vol. 5, no 4, 271-282 p.
Keyword [en]
AMPK, Brown adipose tissue, Hypothalamus, Thyroid hormones, UCP1
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-129906DOI: 10.1016/j.molmet.2016.01.008ISI: 000372823000004PubMedID: 27069867OAI: oai:DiVA.org:su-129906DiVA: diva2:925800
Available from: 2016-05-03 Created: 2016-05-03 Last updated: 2016-05-03Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Csikasz, Robert I.Cannon, BarbaraNedergaard, Jan
By organisation
Department of Molecular Biosciences, The Wenner-Gren Institute
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 103 hits
ReferencesLink to record
Permanent link

Direct link