Change search
ReferencesLink to record
Permanent link

Direct link
Synaptic circuitry of identified neurons in the antennal lobe of Drosophila melanogaster
Show others and affiliations
Number of Authors: 6
2016 (English)In: Journal of Comparative Neurology, ISSN 0021-9967, E-ISSN 1096-9861, Vol. 524, no 9, 1920-1956 p.Article in journal (Refereed) Published
Abstract [en]

In Drosophila melanogaster olfactory sensory neurons (OSNs) establish synapses with projection neurons (PNs) and local interneurons within antennal lobe (AL) glomeruli. Substantial knowledge regarding this circuitry has been obtained by functional studies, whereas ultrastructural evidence of synaptic contacts is scarce. To fill this gap, we studied serial sections of three glomeruli using electron microscopy. Ectopic expression of a membrane-bound peroxidase allowed us to map synaptic sites along PN dendrites. Our data prove for the first time that each of the three major types of AL neurons is both pre- and postsynaptic to the other two types, as previously indicated by functional studies. PN dendrites carry a large proportion of output synapses, with approximately one output per every three input synapses. Detailed reconstructions of PN dendrites showed that these synapses are distributed unevenly, with input and output sites partially segregated along a proximal-distal gradient and the thinnest branches carrying solely input synapses. Moreover, our data indicate synapse clustering, as we found evidence of dendritic tiling of PN dendrites. PN output synapses exhibited T-shaped presynaptic densities, mostly arranged as tetrads. In contrast, output synapses from putative OSNs showed elongated presynaptic densities in which the T-bar platform was supported by several pedestals and contacted as many as 20 postsynaptic profiles. We also discovered synaptic contacts between the putative OSNs. The average synaptic density in the glomerular neuropil was about two synapses/mu m(3). These results are discussed with regard to current models of olfactory glomerular microcircuits across species. J. Comp. Neurol. 524:1920-1956, 2016.

Place, publisher, year, edition, pages
2016. Vol. 524, no 9, 1920-1956 p.
Keyword [en]
Drosophila melanogaster, olfactory system, glomerulus, projection neuron, ultrastructure, synaptic microcircuits
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-130630DOI: 10.1002/cne.23966ISI: 000374382300009PubMedID: 26780543OAI: oai:DiVA.org:su-130630DiVA: diva2:933120
Available from: 2016-06-03 Created: 2016-05-27 Last updated: 2016-06-03Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Cantera, Rafael
By organisation
Department of Zoology
In the same journal
Journal of Comparative Neurology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 10 hits
ReferencesLink to record
Permanent link

Direct link