Quantitative Microplate Assay for Real-Time Nuclease Kinetics
Number of Authors: 2
2016 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 11, no 4, e0154099Article in journal (Refereed) Published
Utilizing the phenomenon of nucleases exposing oligonucleotide phosphate backbones to phosphatases we present a novel quantitative method for kinetics of nuclease catalysis. Inorganic phosphate released from nuclease products by phosphatases could be quantified in real-time by a fluorescent sensor of inorganic phosphate. Two different nucleases were employed, showing the versatility of this assay for multiple turnover label-free nuclease studies.
Place, publisher, year, edition, pages
2016. Vol. 11, no 4, e0154099
Research subject Neurochemistry with Molecular Neurobiology
IdentifiersURN: urn:nbn:se:su:diva-130869DOI: 10.1371/journal.pone.0154099ISI: 000374898500143PubMedID: 27101307OAI: oai:DiVA.org:su-130869DiVA: diva2:934015