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Investigating Membrane Interactions and Structures of CPPs
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Number of Authors: 2
2015 (English)In: Cell-Penetrating Peptides: Methods and Protocols / [ed] Ülo Langel, New York: Springer, 2015, Vol. 1324, 73-87 p.Chapter in book (Refereed)
Abstract [en]

Despite many studies made on cell-penetrating peptides (CPPs), the mechanism of their cellular uptake and endosomal escape has not been completely resolved. This is even more unclear when the CPP is bound either covalently or non-covalently to the cargo molecules. To answer remaining questions, we require a combination of different methods, model systems, and experiments since there is no single method which could give a complete answer to all questions. Biophysical investigations of CPPs have a significant impact on CPP research considering their molecular mechanisms of action. In this chapter, we present different membrane model systems suitable for biophysical studies as well as the basic practical aspects underlying several common biophysical methods and experiments. The methods include fluorescence spectroscopy, circular dichroism spectroscopy, and dynamic light scattering and concern peptide-membrane interactions and vesicle model membrane leakage. We have also described the potential and limitations of biophysical studies on the CPP-membrane interactions and their impact on our understanding of how CPPs mediate the transport of cargoes into living cells.

Place, publisher, year, edition, pages
New York: Springer, 2015. Vol. 1324, 73-87 p.
Series
, Methods in Molecular Biology, ISSN 1064-3745 ; 1324
Keyword [en]
Large unilamellar vesicle, Circular dichroism, Dynamic light scattering, Nuclear magnetic resonance, Bacteriorhodopsin, pH gradient, Calcein leakage, Proton pumping
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-131580DOI: 10.1007/978-1-4939-2806-4_5ISI: 000376037700006PubMedID: 26202263ISBN: 978-1-4939-2805-7ISBN: 978-1-4939-2806-4OAI: oai:DiVA.org:su-131580DiVA: diva2:941308
Available from: 2016-06-22 Created: 2016-06-21 Last updated: 2016-06-22Bibliographically approved

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Gräslund, Astrid
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Department of Biochemistry and Biophysics
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