Change search
ReferencesLink to record
Permanent link

Direct link
Inactivation of Mitochondrial Complex I Induces the Expression of a Twin Cysteine Protein that Targets and Affects Cytosolic, Chloroplastidic and Mitochondrial Function
Show others and affiliations
Number of Authors: 17
2016 (English)In: Molecular Plant, ISSN 1674-2052, E-ISSN 1752-9867, Vol. 9, no 5, 696-710 p.Article in journal (Refereed) Published
Abstract [en]

At12Cys-1 (At5g64400) and At12Cys-2 (At5g09570) are two closely related isogenes that encode small, twin cysteine proteins, typically located in mitochondria. At12Cys-2 transcript is induced in a variety of mutants with disrupted mitochondrial proteins, but an increase in At12Cys protein is only detected in mutants with reduced mitochondrial complex I abundance. Induction of At12Cys protein in mutants that lack mitochondrial complex I is accompanied by At12Cys protein located in mitochondria, chloroplasts, and the cytosol. Biochemical analyses revealed that even single gene deletions, i.e., At12cys-1 or At12cys-2, have an effect on mitochondrial and chloroplast functions. However, only double mutants, i.e., At12cys-1: At12cys-2, affect the abundance of protein and mRNA transcripts encoding translation elongation factors as well as rRNA abundance. Blue native PAGE showed that At12Cys co-migrated with mitochondrial supercomplex I + III. Likewise, deletion of both At12cys-1 and At12cys-2 genes, but not single gene deletions, results in enhanced tolerance to drought and light stress and increased anti-oxidant capacity. The induction and multiple localization of At12Cys upon a reduction in complex I abundance provides a mechanism to specifically signal mitochondrial dysfunction to the cytosol and then beyond to other organelles in the cell.

Place, publisher, year, edition, pages
2016. Vol. 9, no 5, 696-710 p.
Keyword [en]
mitochondria, complex I, retrograde signaling, chloroplast, cytosol
National Category
Biological Sciences
URN: urn:nbn:se:su:diva-131535DOI: 10.1016/j.molp.2016.01.009ISI: 000375958800007PubMedID: 26829715OAI: diva2:945539
Available from: 2016-07-01 Created: 2016-06-21 Last updated: 2016-07-01Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Teixeira, Pedro F.Kmiec, BeataGlaser, Elzbieta
By organisation
Department of Biochemistry and Biophysics
In the same journal
Molecular Plant
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 7 hits
ReferencesLink to record
Permanent link

Direct link