Change search
ReferencesLink to record
Permanent link

Direct link
Differential Response and Priming Dose Effect on the Proteome of Human Fibroblast and Stem Cells Induced by Exposure to Low Doses of Ionizing Radiation
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Show others and affiliations
Number of Authors: 12
2016 (English)In: Radiation Research, ISSN 0033-7587, E-ISSN 1938-5404, Vol. 185, no 3, 299-312 p.Article in journal (Refereed) Published
Abstract [en]

It has been suggested that a mechanistic understanding of the cellular responses to low dose and dose rate may be valuable in reducing some of the uncertainties involved in current risk estimates for cancer- and non-cancer-related radiation effects that are inherited in the linear nothreshold hypothesis. In this study, the effects of low-dose radiation on the proteome in both human fibroblasts and stem cells were investigated. Particular emphasis was placed on examining: 1. the dose-response relationships for the differential expression of proteins in the low-dose range (40-140 mGy) of low-linear energy transfer (LET) radiation; and 2. the effect on differential expression of proteins of a priming dose given prior to a challenge dose (adaptive response effects). These studies were performed on cultured human fibroblasts (VH10) and human adipose-derived stem cells (ADSC). The results from the VH10 cell experiments demonstrated that low-doses of low-LET radiation induced unique patterns of differentially expressed proteins for each dose investigated. In addition, a low priming radiation dose significantly changed the protein expression induced by the subsequent challenge exposure. In the ADSC the number of differentially expressed proteins was markedly less compared to VH10 cells, indicating that ADSC differ in their intrinsic response to low doses of radiation. The proteomic results are further discussed in terms of possible pathways influenced by low-dose irradiation.

Place, publisher, year, edition, pages
2016. Vol. 185, no 3, 299-312 p.
National Category
Biological Sciences Radiology, Nuclear Medicine and Medical Imaging
URN: urn:nbn:se:su:diva-132013DOI: 10.1667/RR14226.1ISI: 000376754000008PubMedID: 26934482OAI: diva2:951596
Available from: 2016-08-09 Created: 2016-07-05 Last updated: 2016-08-09Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Haghdoost, SiamakHarms-Ringdahl, Mats
By organisation
Department of Molecular Biosciences, The Wenner-Gren Institute
In the same journal
Radiation Research
Biological SciencesRadiology, Nuclear Medicine and Medical Imaging

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 1 hits
ReferencesLink to record
Permanent link

Direct link