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Neisseria meningitidis Polynucleotide Phosphorylase Affects Aggregation, Adhesion, and Virulence
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
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Number of Authors: 9
2016 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 84, no 5, 1501-1513 p.Article in journal (Refereed) Published
Abstract [en]

Neisseria meningitidis autoaggregation is an important step during attachment to human cells. Aggregation is mediated by type IV pili and can be modulated by accessory pilus proteins, such as PilX, and posttranslational modifications of the major pilus subunit PilE. The mechanisms underlying the regulation of aggregation remain poorly characterized. Polynucleotide phosphorylase ( PNPase) is a 3'-5' exonuclease that is involved in RNA turnover and the regulation of small RNAs. In this study, we biochemically confirm that NMC0710 is the N. meningitidis PNPase, and we characterize its role in N. meningitidis pathogenesis. We show that deletion of the gene encoding PNPase leads to hyperaggregation and increased adhesion to epithelial cells. The aggregation induced was found to be dependent on pili and to be mediated by excessive pilus bundling. PNPase expression was induced following bacterial attachment to human cells. Deletion of PNPase led to global transcriptional changes and the differential regulation of 469 genes. We also demonstrate that PNPase is required for full virulence in an in vivo model of N. meningitidis infection. The present study shows that PNPase negatively affects aggregation, adhesion, and virulence in N. meningitidis.

Place, publisher, year, edition, pages
2016. Vol. 84, no 5, 1501-1513 p.
National Category
Biological Sciences Microbiology in the medical area
Identifiers
URN: urn:nbn:se:su:diva-131999DOI: 10.1128/IAI.01463-15ISI: 000377106600024PubMedID: 26930706OAI: oai:DiVA.org:su-131999DiVA: diva2:952118
Available from: 2016-08-11 Created: 2016-07-05 Last updated: 2016-08-11Bibliographically approved

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Engman, JakobSigurlásdóttir, SaraSjölinder, HongJonsson, Ann-Beth
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Department of Molecular Biosciences, The Wenner-Gren Institute
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Infection and Immunity
Biological SciencesMicrobiology in the medical area

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