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Anchoring of FRET Sensors-A Requirement for Spatiotemporal Resolution
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
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Number of Authors: 5
2016 (English)In: Sensors, ISSN 1424-8220, E-ISSN 1424-8220, Vol. 16, no 5, 703Article in journal (Refereed) Published
Abstract [en]

FRET biosensors have become a routine tool for investigating mechanisms and components of cell signaling. Strategies for improving them for particular applications are continuously sought. One important aspect to consider when designing FRET probes is the dynamic distribution and propagation of signals within living cells. We have addressed this issue by directly comparing an anchored (taFS) to a non-anchored (naFS) cleavable FRET sensor. We chose a microtubule-associated protein tau as an anchor, as microtubules are abundant throughout the cytosol of cells. We show that tau-anchored FRET sensors are concentrated at the cytoskeleton and enriched in the neurite-like processes of cells, providing high intensity of the total signal. In addition, anchoring limits the diffusion of the sensor, enabling spatiotemporally resolved monitoring of subcellular variations in enzyme activity. Thus, anchoring is an important aspect to consider when designing FRET sensors for deeper understanding of cell signaling.

Place, publisher, year, edition, pages
2016. Vol. 16, no 5, 703
Keyword [en]
apoptosis, caspase, FRET sensor, live cell imaging, neurodegeneration, signal transduction
National Category
Biological Sciences Biomedical Laboratory Science/Technology Analytical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-132518DOI: 10.3390/s16050703ISI: 000378510400074OAI: oai:DiVA.org:su-132518DiVA: diva2:952772
Available from: 2016-08-15 Created: 2016-08-15 Last updated: 2016-08-15Bibliographically approved

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Ivanova, Elena V.Figueroa, Ricardo A.Hallberg, EinarIverfeldt, Kerstin
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