The Troodos ophiolite is an ideal location to investigate the relationships between metal mobility from source areas and the formation of volcanogenic massive sulphide (VMS) deposits. The ophiolite is host to the classic “Cyprus-type” Cu-rich VMS deposits as well as abundant zones of epidosite alteration in the lower sheeted dyke section that are significantly depleted in base metals including Cu and Zn, and are considered to be the source of the metals enriched in the overlying deposits. Previous research indicates that the Troodos VMS deposits are irregularly enriched in Au and related elements As, Sb, and Se, but the behaviour of these elements during the hydrothermal alteration of the Troodos ophiolite hitherto has not been investigated. Analyses of fresh glass samples reveal that the Troodos primitive crust has a similar metal content and distribution to modern-day arc-related environments such as the Manus Basin. Compared to mid-oceanic ridge basalt (MORB), the Troodos primitive crust is enriched in As, Sband Pb most likely due to addition from a subducting slab during crustal formation. During early stages ofmagmatic differentiation (9-3.5 wt.% MgO) Au, As, Sb, Se, Cu, Zn and Pb behave as incompatible elements due to the sulphide-undersaturated nature of the melt. The onset of magnetite crystallisation, however, at ~3.5 wt.% MgO leads to sulphide segregation and depletion of strongly chalcophile elements (Au, Cu and Se) during continued differentiation (<3.5 wt.% MgO) whereas poorly chalcophile elements (As, Sb, Zn and Pb) remain incompatible. These differences in metal behaviour can account for the Curich, Zn-Pb-poor of the Cyprus-type VMS deposits as the source area rocks show high Cu fertility compared to Zn and Pb. Mobilisation of metals during hydrothermal alteration of the Troodos ophiolite is more extensive than observed in hydrothermally altered MORB. Mass balance calculations show that the epidosite zones are significantly depleted in Au (-88±16 %), As (-89±23 %), Sb (-60±12 %), Se (-91±20 %), Cu (-84±18 %), Zn (-63±9 %) and Pb (-60±8 %). Background altered diabase from outside epidosite zones shows similar metal depletions which suggests that the source areas of VMS are not restricted to epidosite zones but are extended to the lower sheeted dyke section. The masses of metals mobilised from a source area of 10.9 km3, (composed of a 5 km3 epidosite zone and 5.9 km3 of background altered diabase) in the Solea graben are 47 t Au, 21 kt As, 1220 t Sb, 3080 t Se, 2.4 Mt Cu, 1.8 Mt Zn and 27 kt Pb. Comparison of metal quantities mobilised from lower sheeted dike section in the Solea graben with those hosted in VMS deposits shows trapping efficiencies ranging from 4 to 37 % indicating that most of the metals is lost by other processes.