Structural studies of a polysaccharide from Vibrio parahaemolyticus strain AN-16000
Number of Authors: 5
2016 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 432, 41-49 p.Article in journal (Refereed) Published
The structure of a polysaccharide from Vibrio parahaemolyticus strain AN-16000 has been investigated. The sugar and absolute configuration analysis revealed D-Glc, D-GalN, D-QuiN and L-FucN as major components. The PS was subjected to dephosphorylation with aqueous 40% HF to obtain an oligosaccharide that was analyzed by H-1 and C-13 NMR spectroscopy. The HR-MS spectrum of the oligosaccharide revealed a pentasaccharide composed of two Glc residues, one QuiNAc and one GalNAc, one FucNAc, as well as a glycerol moiety. The structure of the PS was determined using H-1, C-13, N-15 and P-31 NMR spectroscopy; inter-residue correlations were identified by H-1, C-13-heteronuclear multiple-bond correlation, H-1, H-1-NOESY and H-1, P-31-hetero-TOCSY experiments. The PS backbone has the following teichoic acid-like structure: -> 3)-D-Gro-(1-P-6)-beta-D-Glcp-(1 -> 4)-alpha-L-FucpNAc-(1 -> 3)-beta-D-QuipNAc-(1 -> with a side-chain consisting of alpha-D-Glcp-(1 -> 6)-alpha-D-GalpNAc-(1 -> linked to the O3 position of the FucNAc residue.
Place, publisher, year, edition, pages
2016. Vol. 432, 41-49 p.
Vibrio parahemolyticus, NMR, Dephosphorylation, Glycerol-1-phosphate
IdentifiersURN: urn:nbn:se:su:diva-133271DOI: 10.1016/j.carres.2016.06.004ISI: 000380682800007PubMedID: 27392309OAI: oai:DiVA.org:su-133271DiVA: diva2:957987