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Determination of metoprolol enantiomers in human plasma and saliva samples utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry
Stockholm University, Faculty of Science, Department of Environmental Science and Analytical Chemistry. Damanhour University, Egypt.
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Number of Authors: 6
2016 (English)In: BMC Biomedical chromotography, ISSN 0269-3879, E-ISSN 1099-0801, Vol. 30, no 8, 1309-1317 p.Article in journal (Refereed) Published
Abstract [en]

A sensitive, accurate and reliable bioanalytical method for the enantioselective determination of metoprolol in plasma and saliva samples utilizing liquid chromatography-electrospray ionization tandem mass spectrometry was developed and validated. Human plasma and saliva samples were pretreated by microextraction by packed sorbent (MEPS) prior to analysis. A new MEPS syringe form with two inputs was used. Metoprolol enantiomers and internal standard pentycaine (IS) were eluted from MEPS sorbent using isopropanol after removal of matrix interferences using aliquots of 5% methanol in water. Complete separation of metoprolol enantiomers was achieved on a Cellulose-SB column (150x4.6mm, 5m) using isocratic elution with mobile phase 0.1% ammonium hydroxide in hexane-isopropanol (80:20, v/v) with a flow rate of 0.8mL/min. A post-column solvent-assisted ionization was applied to enhance metoprolol ionization signal in positive mode monitoring (+ES) using 0.5% formic acid in isopropanol at a flow rate of 0.2mL/min. The total chromatographic run time was 10min for each injection. The detection of metoprolol in plasma and saliva samples was performed using triple quadrupole tandem mass spectrometer in +ES under the following mass transitions: m/z 268.0872.09 for metoprolol and m/z 303.3154.3 for IS. The linearity range was 2.5-500ng/mL for both R- and S-metoprolol in plasma and saliva. The limits of detection and quantitation for both enantiomers were 0.5 and 2.5ng/mL respectively, in both matrices (plasma and saliva). The intra- and inter-day precisions were presented in terms of RSD values for replicate analysis of quality control samples and were <5%; the accuracy of determinations varied from 96 to 99%. The method was able to determine the therapeutic levels of metoprolol enantiomers in both human plasma and saliva samples successfully, which can aid in therapeutic drug monitoring in clinical laboratories.

Place, publisher, year, edition, pages
2016. Vol. 30, no 8, 1309-1317 p.
Keyword [en]
metoprolol enantiomers, chiral chromatography, tandem mass spectrometry, therapeutic drug monitoring, plasma, saliva
National Category
Biological Sciences Chemical Sciences Pharmaceutical Sciences
URN: urn:nbn:se:su:diva-133381DOI: 10.1002/bmc.3685ISI: 000379971200020PubMedID: 26766521OAI: diva2:967589
Available from: 2016-09-09 Created: 2016-09-06 Last updated: 2016-09-09Bibliographically approved

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Elmongy, HatemColmsjö, AndersAbdel-Rehim, Mohamed
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