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A Partially Purified Acinetobacter baumannii Phage Preparation Exhibits no Cytotoxicity in 3T3 Mouse Fibroblast Cells
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
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Number of Authors: 5
2016 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 7, 1198Article in journal (Refereed) Published
Abstract [en]

A surge in the level and scale of antibiotic resistance has prompted renewed interest in the application of bacteriophages to treat bacterial infections. However, concerns still exist over their efficacy and safety. Acinetobacter baurnannii phage BS46, a member of the family Myoviridae, has previously been shown to be effective in murine models. The cytotoxic effect of this phage was evaluated in mouse fibroblast 3T3 cells using four different assays: trypan blue; staining with Hoechst and propidium iodide; lactate dehydrogenase release; and the MTS assay. The addition of phage concentrations up to 2 x 10(9) pfu/mL showed little to no impact on the viability of 3T3 cells after 24 h exposure using the different assays. This study demonstrates that phage BS46 is non-cytotoxic to 3T3 cells using four different assays and that appropriate quality assurance protocols for phage therapeutics are required.

Place, publisher, year, edition, pages
2016. Vol. 7, 1198
Keyword [en]
bacteriophage, cytotoxicity, Acinetobacter baumannii, lactate dehydrogenase, MTS assay, trypan blue, hoechst stain, propidium iodide
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-133373DOI: 10.3389/fmicb.2016.01198ISI: 000380656000001PubMedID: 27536286OAI: oai:DiVA.org:su-133373DiVA: diva2:967816
Available from: 2016-09-09 Created: 2016-09-06 Last updated: 2016-09-09Bibliographically approved

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Cooper, Callum J.
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Department of Molecular Biosciences, The Wenner-Gren Institute
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