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  • 1.
    Olsson, Petter
    et al.
    Stockholm University, Faculty of Science, Department of Environmental Science and Analytical Chemistry.
    Holmbäck, Jan
    Stockholm University, Faculty of Science, Department of Analytical Chemistry.
    Herslöf, Bengt
    Stockholm University, Faculty of Science, Department of Analytical Chemistry.
    A single step reversed-phase high performance liquid chromatography separation of polar and non-polar lipids2014In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1369, p. 105-115Article in journal (Refereed)
    Abstract [en]

    This paper reports a simple chromatographic system to separate lipids classes as well as their molecular species. By the use of phenyl coated silica as stationary phase in combination with a simple mobile phase consisting of methanol and water, all tested lipid classes elute within 30min. Furthermore, a method to accurately predict retention times of specific lipid components for this type of chromatography is presented. Common detection systems were used, namely evaporative light scattering detection (ELSD), charged aerosol detection (CAD), electrospray mass spectrometry (ESI-MS), and UV detection.

  • 2.
    Olsson, Petter
    et al.
    Stockholm University, Faculty of Science, Department of Analytical Chemistry.
    Holmbäck, Jan
    Stockholm University, Faculty of Science, Department of Analytical Chemistry.
    Herslöf, Bengt
    Stockholm University, Faculty of Science, Department of Analytical Chemistry.
    Separation of Lipid Classes by HPLC on a Cyanopropyl Column2012In: Lipids, ISSN 0024-4201, E-ISSN 1558-9307, Vol. 47, no 1, p. 93-99Article in journal (Refereed)
    Abstract [en]

    A new method for the separation and identification of lipid classes by normal-phase HPLC on a cyanopropyl column is described. The use of a simple binary gradient, with toluene as a component, provided a rapid separation of non-polar as well as phospholipid classes. The inherent small differences in performances between possible non-polar eluent components of the gradient, such as hexane, heptane, and iso-octane, had a pronounced impact on retention times for individual phospholipid classes. Separation of molecular species within a lipid class could also be observed.

  • 3.
    Olsson, Petter
    et al.
    Stockholm University, Faculty of Science, Department of Analytical Chemistry.
    Holmbäck, Jan
    Stockholm University, Faculty of Science, Department of Analytical Chemistry.
    Nilsson, Ulrika
    Stockholm University, Faculty of Science, Department of Analytical Chemistry.
    Herslöf, Bengt
    Stockholm University, Faculty of Science, Department of Analytical Chemistry.
    Separation And Identification Of Lipid Classes By Normal Phase Lc-Esi/Ms/Ms On A Cyanopropyl Column2014In: European Journal of Lipid Science and Technology, ISSN 1438-7697, E-ISSN 1438-9312, Vol. 116, no 5, p. 653-658Article in journal (Refereed)
    Abstract [en]

    In order to establish a versatile and convenient method for the analysis of lipids, electrospray ionization tandem mass spectrometry (ESI-MS/MS) was applied to a HPLC separation on a cyanopropyl-bonded stationary phase. A binary gradient mobile phase system consisting of hexane, toluene, methanol and a stable electrospray yielding sodium adduct ions could be used to generate specific product ions in MS/MS mode. By applying the LC/ESI-MS/MS method on an egg yolk sample, 29 different molecular species of phosphatidylethanolamines, phosphatidylcholines, and lysophosphatidylcholines could be detected within 25 min.

  • 4.
    Pauter, Anna M.
    et al.
    Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
    Olsson, Petter
    Stockholm University, Faculty of Science, Department of Analytical Chemistry.
    Asadi, Abolfazl
    Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
    Herslöf, Bengt
    Stockholm University, Faculty of Science, Department of Analytical Chemistry.
    Csikasz, Robert I.
    Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
    Zadravec, Damir
    Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
    Jacobsson, Anders
    Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
    Elovl2 ablation demonstrates that systemic DHA is endogenously produced and is essential for lipid homeostasis in mice2014In: Journal of Lipid Research, ISSN 0022-2275, E-ISSN 1539-7262, Vol. 55, no 4, p. 718-728Article in journal (Refereed)
    Abstract [en]

    The potential role of endogenously synthesized polyunsaturated fatty acids (PUFAs) is a highly overlooked area. Elongation of very long chain (ELOVL) fatty acids in mammals is catalyzed by the ELOVL enzymes to which the PUFA elongase ELOVL2 belongs. To determine its in vivo function, we have investigated how ablation of ELOVL2, which is highly expressed in liver, affects hepatic lipid composition and function in mice. The Elovl2 ablated mice displayed substantial decreased levels of 22:6(n3), docosahexaenoic acid (DHA), and 22:5(n6), docosapentaenoic acid (DPAn6), followed by an accumulation of 22:5(n3) and 22:4(n6) in both liver and serum showing that ELOVL2 primarily controls the elongation process of PUFAs with 22 carbons to produce 24 carbon precursors for DHA and DPA(n6) formation in vivo. The impaired PUFA levels positively influenced hepatic levels of the key lipogenic transcriptional regulator sterol regulatory element binding protein 1c (SREBP1c) as well as its downstream target genes. Surprisingly, the Elovl2 ablated mice were resistant against hepatic steatosis and diet induced weight gain implying that hepatic DHA synthesis via ELOVL2, except controlling de novo lipogenesis, also regulates lipid storage and fat mass expansion in an SREBP1c independent fashion. The changes in fatty acid metabolism were reversed by dietary supplementation with DHA.

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