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  • 1.
    Bárány-Wallje, Elsa
    et al.
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. biofysik.
    Andersson, August
    Gräslund, Astrid
    Mäler, Lena
    NMR solution structure and position of transportan in neutral phospholipid bicelles.2004In: FEBS Lett, ISSN 0014-5793, Vol. 567, no 2-3, p. 265-9Article in journal (Refereed)
    Abstract [en]

    Transportan is a chimeric cell-penetrating peptide constructed from the peptides galanin and mastoparan, which has the ability to internalize living cells carrying a hydrophilic load. In this study, we have determined the NMR solution structure and investigated the position of transportan in neutral bicelles. The structure revealed a well-defined -helix in the C-terminal mastoparan part of the peptide and a weaker tendency to form an -helix in the N-terminal domain. The position of the peptide in relation to the membrane, as studied by adding paramagnetic probes, shows that the peptide lies parallel to, and in the head-group region of the membrane surface. This result is supported by amide proton secondary chemical shifts.

  • 2.
    Bárány-Wallje, Elsa
    et al.
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Gaur, Jugnu
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Lundberg, Pontus
    Stockholm University, Faculty of Science, Department of Neurochemistry.
    Langel, Ülo
    Stockholm University, Faculty of Science, Department of Neurochemistry.
    Gräslund, Astrid
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Differential membrane perturbation caused by the cell penetrating peptide Tp10 depending on attached cargo2007In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 581, no 13, p. 2389-2393Article in journal (Refereed)
    Abstract [en]

    The membrane leakage caused by the cell penetrating peptide Tp10, a variant of transportan, was studied in large unilamellar vesicles with the entrapped fluorophore calcein. The vesicles were composed of zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine. A significant decrease in membrane leakage was found when the 55 kDa streptavidin protein was attached to Tp10. When a 5.4 kDa peptide nucleic acid molecule was attached, the membrane leakage was comparable to that caused by Tp10 alone. The results suggest that direct membrane effects may cause membrane translocation of Tp10 alone and of smaller complexes, whereas these effects do not contribute for larger cargoes.

  • 3.
    Bárány-Wallje, Elsa
    et al.
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Gräslund, Astrid
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Mäler, Lena
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Structure and dynamics of galanin in phospholipid bicelles2007Conference paper (Other (popular science, discussion, etc.))
  • 4.
    Bárány-Wallje, Elsa
    et al.
    Stockholm University.
    Keller, Sandro
    Serowy, Steffen
    Geibel, Sebastian
    Pohl, Peter
    Bienert, Michael
    Dathe, Margitta
    A critical reassessment of penetratin translocation across lipid membranes.2005In: Biophys J, ISSN 0006-3495, Vol. 89, no 4, p. 2513-21Article in journal (Refereed)
    Abstract [en]

    Penetratin is a short, basic cell-penetrating peptide able to induce cellular uptake of a vast variety of large, hydrophilic

    cargos. We have reassessed the highly controversial issue of direct permeation of the strongly cationic peptide across negatively charged lipid membranes. Confocal laser scanning microscopy on rhodamine-labeled giant vesicles incubated with carboxyfluorescein-labeled penetratin yielded no evidence of transbilayer movement, in contradiction to previously reported results. Confocal fluorescence spectroscopy on black lipid membranes confirmed this finding, which was also not affected by application of a transmembrane electric potential difference.

    A novel dialysis assay based on tryptophan absorbance and fluorescence spectroscopy demonstrated that the permeability of small and large unilamellar vesicles to penetratin is,<10^-13m/s.

    Taken together, the results show that penetratin is not capable of overcoming model membrane systems irrespective of the bilayer curvature or the presence of a transmembrane voltage. Thus, direct translocation across the hydrophobic core of the plasmamembrane cannot account for the efficient uptake of penetratin into live cells, which is in accord with recent in vitro studies underlining the importance of endocytosis in the internalization process of cationic cell-penetrating peptides.

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