Ändra sökning
Avgränsa sökresultatet
1234 1 - 50 av 179
RefereraExporteraLänk till träfflistan
Permanent länk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Träffar per sida
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sortering
  • Standard (Relevans)
  • Författare A-Ö
  • Författare Ö-A
  • Titel A-Ö
  • Titel Ö-A
  • Publikationstyp A-Ö
  • Publikationstyp Ö-A
  • Äldst först
  • Nyast först
  • Skapad (Äldst först)
  • Skapad (Nyast först)
  • Senast uppdaterad (Äldst först)
  • Senast uppdaterad (Nyast först)
  • Disputationsdatum (tidigaste först)
  • Disputationsdatum (senaste först)
  • Standard (Relevans)
  • Författare A-Ö
  • Författare Ö-A
  • Titel A-Ö
  • Titel Ö-A
  • Publikationstyp A-Ö
  • Publikationstyp Ö-A
  • Äldst först
  • Nyast först
  • Skapad (Äldst först)
  • Skapad (Nyast först)
  • Senast uppdaterad (Äldst först)
  • Senast uppdaterad (Nyast först)
  • Disputationsdatum (tidigaste först)
  • Disputationsdatum (senaste först)
Markera
Maxantalet träffar du kan exportera från sökgränssnittet är 250. Vid större uttag använd dig av utsökningar.
  • 1. Al-Anati, Lauy
    et al.
    Viluksela, Matti
    Strid, Anna
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi. Swedish Toxicology Sciences Research Center (Swetox), Sweden.
    Andersson, Patrik L.
    Stenius, Ulla
    Högberg, Johan
    Hydroxyl metabolite of PCB 180 induces DNA damage signaling and enhances the DNA damaging effect of benzo[a]pyrene2015Ingår i: Chemico-Biological Interactions, ISSN 0009-2797, E-ISSN 1872-7786, Vol. 239, s. 164-173Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Non-dioxin-like (NDL) polychlorinated biphenyls (PCBs) and their hydroxyl metabolites (OH-PCBs) are ubiquitous environmental contaminants in human tissues and blood. The toxicological impact of these metabolites is poorly understood. In this study rats were exposed to ultrapure PCB180 (10-1000 mg/kg bw) for 28 days and induction of genotoxic stress in liver was investigated. DNA damage signaling proteins (pChk1Ser317 and gamma H2AXSer319) were increased dose dependently in female rats. This increase was paralleled by increasing levels of the metabolite 3'-OH-PCB180. pChk1 was the most sensitive marker. In in vitro studies HepG2 cells were exposed to 1 mu M of PCB180 and 3'-OH-PCB180 or the positive control benzo[a]pyrene (BaP, 5 mu M). 3'-OH-PCB180, but not PCB180, induced CYP1A1 mRNA and gamma H2AX. CYP1A1 mRNA induction was seen at 1 h, and gamma H2AX at 3 h. The anti-oxidant N-Acetyl-L-Cysteine (NAC) completely prevented, and 17 beta-estradiol amplified the gamma H2AX induction by 3'-OH-PCB180. As 3'-OH-PCB180 induced CYP1A1, a major BaP-metabolizing and activating enzyme, interactions between 3'-OH-PCB180 and BaP was also studied. The metabolite amplified the DNA damage signaling response to BaP. In conclusion, metabolism of PCB180 to its hydroxyl metabolite and the subsequent induction of CYP1A1 seem important for DNA damage induced by PCB180 in vivo. Amplification of the response with estradiol may explain why DNA damage was only seen in female rats.

  • 2. Alexander, Jan
    et al.
    Benford, Diane
    Boobis, Alan
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Scientific Opinion on Hexabromocyclododecanes (HBCDDs) in Food2011Ingår i: EFSA Journal, ISSN 1831-4732, Vol. 9, nr 7, s. 2296-Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    EFSA was asked by the European Commission to deliver a scientific opinion on hexabromocyclododecanes (HBCDDs) in food. HBCDDs are additive flame retardants primarily used in expanded and extruded polystyrene applied as construction and packing materials, and in textiles. Technical HBCDD predominantly consists of three stereoisomers (α-, β- and γ-HBCDD). Also δ- and ε-HBCDD may be present but at very low concentrations. HBCDDs are present in the environment and likewise in biota and in food and feed. Data from the analysis of HBCDDs in 1,914 food samples were provided to EFSA by seven European countries, covering the period from 2000 to 2010. The Panel on Contaminants in the Food Chain (CONTAM Panel) selected α-, β- and γ-HBCDD to be of primary interest. Since all toxicity studies were carried out with technical HBCDD, a risk assessment of individual stereoisomers was not possible. Main targets were the liver, thyroid hormone homeostasis and the reproductive, nervous and immune systems. HBCDDs are not genotoxic. The CONTAM Panel identified neurodevelopmental effects on behaviour as the critical endpoint, and derived a benchmark dose lower confidence limit for a benchmark response of 10 % (BMDL10) of 0.79 mg/kg body weight. Due to the limitations and uncertainties in the current data base, the CONTAM Panel concluded that it was inappropriate to use this BMDL to establish a health based guidance value, and instead used a margin of exposure (MOE) approach for the health risk assessment of HBCDDs. Since elimination characteristics of HBCDDs in animals and humans differ, the Panel used the body burden as starting point for the MOE approach. The CONTAM Panel concluded that current dietary exposure to HBCDDs in the European Union does not raise a health concern. Also additional exposure, particularly of young children, to HBCDDs from house dust is unlikely to raise a health concern

  • 3. Alexander, Jan
    et al.
    Benford, Diane
    Boobis, Alan
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Scientific Opinion on Polybrominated Diphenyl Ethers (PBDEs) in Food2011Ingår i: EFSA Journal, ISSN 1831-4732, Vol. 9, nr 5, s. 2156-Artikel i tidskrift (Övrigt vetenskapligt)
  • 4. Alexander, Jan
    et al.
    Benford, Diane
    Boobis, Alan
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Scientific Opinion on Tetrabromobisphenol A (TBBPA) and its derivatives in food: EFSA Panel on Contaminants in the Food Chain (CONTAM)2011Ingår i: EFSA Journal, ISSN 1831-4732, Vol. 9, nr 12, s. 2477-Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    EFSA was asked by the European Commission to deliver a scientific opinion on tetrabromobisphenol A (TBBPA) and its derivatives in food. TBBPA and its derivatives are widely used as flame retardants. TBBPA is primarily used as reactive flame retardant covalently bound to epoxy and polycarbonate resins. TBBPA derivatives are used as either reactive or additive intermediates in polymer manufacture. Data from the analysis of TBBPA in 344 food samples were submitted to EFSA by two European countries (Norway and Spain), covering the period from 2007 to 2010. All samples were in the food group “Fish and other seafood”, and all analytical results were reported as less than the limit of quantification (LOQ) (about 1 ng/g wet weight). Toxicological studies with TBBPA have been carried out using different experimental designs with single or repeated administration during gestation, postnatally or in adulthood. The main target is thyroid hormone homeostasis. TBBPA is not genotoxic. There are no indications that TBBPA might be carcinogenic. The Panel on Contaminants in the Food Chain (CONTAM Panel) identified a lower confidence limit for a benchmark response of 10 % (BMDL10) of 16 mg/kg b.w. reported for changes in thyroid hormones as the critical reference point. Due to the limitations and uncertainties in the database, the CONTAM Panel concluded that it was inappropriate to use this BMDL to establish a health based guidance value, and therefore used a margin of exposure (MOE) approach for the health risk assessment of TBBPA. In view of the large MOEs, the CONTAM Panel concluded that current dietary exposure to TBBPA in the European Union does not raise a health concern. Also exposure of infants via human milk does not raise a health concern. Additional exposure, particularly of young children, to TBBPA from house dust is unlikely to raise a health concern.

  • 5. Alm, Henrik
    et al.
    Scholz, Birger
    Kultima, Kim
    Nilsson, Anna
    Andren, Per E.
    Savitski, Mikhail M.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Stigson, Michael
    Fex-Svenningsen, Asa
    Dencker, Lennart
    In Vitro Neurotoxicity of PBDE-99: Immediate and Concentration-Dependent Effects on Protein Expression in Cerebral Cortex Cells2010Ingår i: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 9, nr 3, s. 1226-1235Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Polybrominated diphenyl ethers (PBDEs) are commonly used flame retardants in various consumer products. Pre- and postnatal exposure to congeners of PBDEs disrupts normal brain development in rodents. Two-dimensional difference gel electrophoresis (2D-DIGE) was used to analyze concentration-dependent differences in protein expression in cultured cortical cells isolated from rat fetuses (GD 21) after 24 h exposure to PBDE-99 (3, 10, or 30 mu M). Changes on a post-translational level were studied using a 1 h exposure to 30 mu M PBDE-99. The effects of 24 h exposure to 3 and 30 mu M PBDE-99 on mRNA levels were measured using oligonucleotide microarrays. A total of 62, 46, and 443 proteins were differentially expressed compared to controls after 24 h of exposure to 3, 10, and 30 mu M PDBE-99, respectively. Of these, 48, 43, and 238 proteins were successfully identified, respectively. We propose that the biological effects of low-concentration PBDE-99 exposure are fundamentally different than effects of high-concentration exposure. Low-dose PBDE-99 exposure induced marked effects on cytoskeletal proteins, which was not correlated to cytotoxicity or major morphological effects, suggesting that other more regulatory aspects of cytoskeletal functions may be affected. Interestingly, 0.3 and 3 mu M, but not 10 or 30 mu M increased the expression of phosphorylated (active) Gap43, perhaps reflecting effects on neurite extension processes.

  • 6.
    Asp, V
    et al.
    Department of Environmental Toxicology, Uppsala University.
    Cantillana, T
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Brandt, I
    Department of Environmental Toxicology, Uppsala University.
    Chiral effects in adrenocorticolytic action of o,p'-DDD (mitotane) in human adrenal cells2010Ingår i: Xenobiotica, ISSN 0049-8254, E-ISSN 1366-5928, Vol. 40, nr 3, s. 177-183Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    1. Adrenocortical carcinoma (ACC) is a rare malignant disease with poor prognosis. The main pharmacological choice, o,p'-DDD (mitotane), produces severe adverse effects. 2. Since o,p'-DDD is a chiral molecule and stereoisomers frequently possess different pharmacokinetic and/or pharmacodynamic properties, we isolated the two o,p'-DDD enantiomers, (R)-(+)-o,p'-DDD and (S)-(-)-o,p'-DDD, and determined their absolute structures. 3. The effects of each enantiomer on cell viability and on cortisol and dehydroepiandrosterone (DHEA) secretion in the human adrenocortical cell line H295R were assessed. We also assayed the o, p'-DDD racemate and the m,p'- and p,p'-isomers. 4. The results show small but statistically significant differences in activity of the o, p'-DDD enantiomers for all parameters tested. The three DDD isomers were equally potent in decreasing cell viability, but p, p'- DDD affected hormone secretion slightly less than the o,p'- and m,p'-isomers. 5. The small chiral differences in direct effects on target cells alone do not warrant single enantiomer administration, but might reach importance in conjunction with possible stereochemical effects on pharmacokinetic processes in vivo.

  • 7.
    Asplund, L
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Löfstrand, K
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Malmvärn, A
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Nylund, K
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för tillämpad miljövetenskap (ITM).
    Eriksson, U
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    OH-PBDEs and MeO-PBDEs in swedish marine and fresh water environment- an overview2010Ingår i: Organohalogen Compounds, 2010Konferensbidrag (Övrigt vetenskapligt)
  • 8.
    Athanasiadou, Maria
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Marsh, Göran
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Athanassiadis, Ioannis
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Asplund, Lillemor
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Gas chromatography and mass spectrometry of methoxylated polybrominated diphenyl ethers (MeO-PBDEs).2006Ingår i: J Mass Spectrom, ISSN 1076-5174, Vol. 41, nr 6, s. 790-801Artikel i tidskrift (Övrigt vetenskapligt)
  • 9.
    Awad, Raed
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap. Swedish Environmental Research Institute (IVL), Sweden.
    Zhou, Yihui
    Nyberg, Elisabeth
    Namazkar, Shahla
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap.
    Yongning, Wu
    Xiao, Qianfen
    Sun, Yaije
    Zhu, Zhiliang
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap. Tongji University, China; Örebro University, Sweden.
    Benskin, Jonathan P.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap.
    Emerging per- and polyfluoroalkyl substances (PFAS) in human milk from Sweden and China2020Ingår i: Environmental Science: Processes & Impacts, ISSN 2050-7887, E-ISSN 2050-7895, Vol. 22, nr 10, s. 2023-2030Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Twenty per- and polyfluoroalkyl substances (PFAS) were determined in human milk from residents of three Chinese cities (Shanghai, Jiaxing, and Shaoxing; [n= 10 individuals per city]), sampled between 2010 and 2016. These data were compared to a combination of new and previously reported PFAS concentrations in human milk from Stockholm, Sweden, collected in 2016 (n= 10 individuals). Across the three Chinese cities, perfluorooctanoate (PFOA; sum isomers), 9-chlorohexadecafluoro-3-oxanone-1-sulfonic acid (9Cl-PF3ONS; also known as 6:2 Cl-PFESA or by its trade name F53-B), and perfluorooctane sulfonate (PFOS; sum isomers) occurred at the highest concentrations among all PFAS (up to 411, 976, and 321 pg mL(-1), respectively), while in Stockholm, PFOA and PFOS were dominant (up to 89 and 72 pg mL(-1), respectively). 3H-Perfluoro-3-[(3-methoxy-propoxy)propanoic acid] (ADONA) was intermittently detected but at concentrations below the method quantification limit (i.e.<10 pg mL(-1)) in Chinese samples, and was non-detectable in Swedish milk. The extremely high concentrations of F53-B in Chinese milk suggest that human exposure assessments focused only on legacy substances may severely underestimate overall PFAS exposure in breastfeeding infants.

  • 10. Barouki, Robert
    et al.
    Kogevinas, Manolis
    Audouze, Karine
    Belesova, Kristine
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap.
    Birnbaum, Linda
    Boekhold, Sandra
    Denys, Sebastien
    Desseille, Celine
    Drakvik, Elina
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap.
    Frumkin, Howard
    Garric, Jeanne
    Destoumieux-Garzon, Delphine
    Haines, Andrew
    Huss, Anke
    Jensen, Genon
    Karakitsios, Spyros
    Klanova, Jana
    Koskela, Iida-Maria
    Laden, Francine
    Marano, Francelyne
    Matthies-Wiesler, Eva Franziska
    Morris, George
    Nowacki, Julia
    Paloniemi, Riikka
    Pearce, Neil
    Peters, Annette
    Rekola, Aino
    Sarigiannis, Denis
    Šebková, Katerinaa
    Slama, Remy
    Staatsen, Brigit
    Tonne, Cathryn
    Vermeulen, Roel
    Vineis, Paolo
    The COVID-19 pandemic and global environmental change: Emerging research needs2021Ingår i: Environment International, ISSN 0160-4120, E-ISSN 1873-6750, Vol. 146, artikel-id 106272Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The outbreak of COVID-19 raised numerous questions on the interactions between the occurrence of new infections, the environment, climate and health. The European Union requested the H2020 HERA project which aims at setting priorities in research on environment, climate and health, to identify relevant research needs regarding Covid-19. The emergence and spread of SARS-CoV-2 appears to be related to urbanization, habitat destruction, live animal trade, intensive livestock farming and global travel. The contribution of climate and air pollution requires additional studies. Importantly, the severity of COVID-19 depends on the interactions between the viral infection, ageing and chronic diseases such as metabolic, respiratory and cardiovascular diseases and obesity which are themselves influenced by environmental stressors. The mechanisms of these interactions deserve additional scrutiny. Both the pandemic and the social response to the disease have elicited an array of behavioural and societal changes that may remain long after the pandemic and that may have long term health effects including on mental health. Recovery plans are currently being discussed or implemented and the environmental and health impacts of those plans are not clearly foreseen. Clearly, COVID-19 will have a longlasting impact on the environmental health field and will open new research perspectives and policy needs.

  • 11.
    Bastos, Patricia Moreira
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Eriksson, Johan
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Green, Nicholas
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    A standardized method for assessment of oxidative transformations of brominated phenols in water.2008Ingår i: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 70, nr 7, s. 1196-202Artikel i tidskrift (Refereegranskat)
  • 12.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Alla kemister behöver miljökemin2010Ingår i: Kemivärlden Biotech med Kemisk Tidskrift, Vol. 10, s. 59-Artikel i tidskrift (Refereegranskat)
  • 13.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    BFR Exposure: Air, particles and food matters2007Ingår i: 4th International Workshop on Brominated Flame Retardants: BFR 2007 Amsterdam, 2007Konferensbidrag (Övrig (populärvetenskap, debatt, mm))
  • 14.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Foreword2012Ingår i: Hormone-Disruptive Chemical Contaminants in Food / [ed] Ingemar Pongratz; Linda Bergander, London: Royal Society of Chemistry, 2012, s. v-viiKapitel i bok, del av antologi (Övrigt vetenskapligt)
  • 15.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Hur förgiftad är vår miljö?2010Ingår i: Miljöforskning; Formas tidning för ett hållbart samhälle, Vol. 4, s. 18-21Artikel i tidskrift (Refereegranskat)
  • 16.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Perspective on recent developments of persistent and bioaccumulative BFRs2008Ingår i: BFR 2008: 10th Annual workshop on brominated flame retardants, 2008, s. 9-Konferensbidrag (Övrig (populärvetenskap, debatt, mm))
  • 17.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Studies on some persistent environmental contaminants: synthesis of chlorinated [¹⁴C] biphenyls and of methyl sulphides and/or methyl sulphones derived from DDE, polychlorobiphenyls and hexachlorobenzene : studies on the in vivo transformation of certain chlorinated biphenyls and hexachlorobenzene to sulphurcontaining metabolites1980Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
  • 18.
    Bergman, Åke
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi. Institutionen för Naturvetenskap och Teknik, Örebro universitet, Sweden; College of Environmental Science and Engineering, Tongji University, China.
    Bignert, AndersHuang, QinghuiCollege of Environmental Science and Engineering, Tongji University, China.Qiu, YanlingCollege of Environmental Science and Engineering, Tongji University, China.Yin, DaqiangCollege of Environmental Science and Engineering, Tongji University, China.
    长三角地区化学品污染与挑战: 经验与启示2019Samlingsverk (redaktörskap) (Övrigt vetenskapligt)
    Abstract [en]

    Chemical Pollution — Challenges in the Yangtze River Delta: Communication Brief is the second and final scientific report of the Chemstrres project. This book covers the results of the cooperation in relation to scientific output, collaborative results and interaction and higher educational achievements including the construction of joint facilities, the Yangtze Environmental Specimen Bank and extended science based exchange. The book is a follow up on Chemical Pollution — Challenges in the Yangtze River Delta (DiVA, id: diva2:1138019). Main results of the ten subprojects performed are given in the book, addressing: i. ecotoxicology studies with the pond snail; ii: on chlorinated paraffins and iii: establishment of the pond snail as a research monitoring species in the Yangtze River Delta. Further. iv: drinking water contaminants were studied in a screening project; v: pollutants in sewage sludge as a mirror of human activities, as well; vi: assessments of persistent and bioaccumulative compounds in wildlife from the Yangtze River Delta were performed; vii: heron eggs were analysed to determine the environmental quality in the region as well as viii:  monitoring of pollutants in mothers’ milk in the Yangtze River Delta, Sweden and Norway were performed. Finally, ix: investigations were carried out to determine optimal fish species for research monitoring in the Yangtze River Delta and in a subproject number x: chemical pollutants were assessed in indoor dust. Reference to 45 authentic peer reviewed scientific articles are presented in the book.

    Ladda ner fulltext (pdf)
    长三角地区化学品污染与挑战
    Ladda ner fulltext (epub)
    长三角地区化学品污染与挑战
    Ladda ner (jpg)
    Omslagsframsida
  • 19.
    Bergman, Åke
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi. Swedish toxicology sciences research center, Sweden; Karolinska Institutet, Sweden; Tongji University, China.
    Bignert, AndersQiu, YanlingStockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi. Tongji University, China.Yin, GeStockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi.
    Chemical Pollution - Challenges in the Yangtze River Delta, China: A 2017 Sino-Swedish Research Report2017Samlingsverk (redaktörskap) (Övrigt vetenskapligt)
    Abstract [en]

    Over time China became the globally most important manufacturer of chemicals and inherited the pollution problems with production and use of chemicals. The Yangtze River Delta is very much a central area for the chemical production and for manufacturing of a variety of chemical products, materials and goods. A science based cooperation between researchers from Tongji University, Stockholm University and the Swedish Museum of Natural History started to develop in the first decade of the present century. The cooperation was aimed to improve the understanding of chemical pollutants and their influence on wildlife and humans in the Yangtze River Delta area, to generate novel data and establish advanced chemical monitoring programs.

    Chemical Pollution — Challenges in the Yangtze River Delta is the first scientific report of the Chemstrres project, "Swedish-Chinese chemical pollution stress and risks research program in the Yangtze River Delta region". The project has been funded by the Swedish Research Council. This book covers the essentials of the natural, social, economic, and chemical environments of the Yangtze River Delta, as well as an up-to-date, introduction of the research activities and highlights within Chemstrres. The book is aimed to attract readers from all sectors of society; vivid graphics and diagrams can be found throughout the text. Both Chemstrres project and this book are expected to bring scientists and decision makers closer together, to enable science based management for improved human health and environmental prosperity. 

    Ladda ner fulltext (pdf)
    Chemical Pollution - Challenges in the Yangtze River Delta, China
    Ladda ner fulltext (epub)
    Chemical Pollution - Challenges in the Yangtze River Delta, China
    Ladda ner (jpg)
    Omslagsframsida
  • 20.
    Bergman, Åke
    et al.
    Karolinska Institutet, Sweden.
    Drakvik, Elina
    Karolinska Institutet, Sweden.
    Rudén, Christina
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi.
    Gennings, Chris
    Mount Sinai School of Medicine, New York, USA.
    EDC-MixRisk Policiy Brief2019Övrigt (Övrigt vetenskapligt)
    Ladda ner fulltext (pdf)
    EDC-MixRisk Policy Brief
  • 21.
    Bergman, Åke
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Lindgren, Torsten
    Smedje, Greta
    Jakobsson, Kristina
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Athanassiadis, Ioannis
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Athanasiadou, Maria
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Meyer, Edgar
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    PBDEs and non-PBDEs in aircraft cabin and cockpit air and dust2010Ingår i: Organohalogen Compounds, 2010Konferensbidrag (Övrigt vetenskapligt)
  • 22.
    Bergman, Åke
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Rydén, Andreas
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Law, Robin J.
    de Boer, Jacob
    Covaci, Adrian
    Alaee, Mehran
    Birnbaum, Linda
    Petreas, Myrto
    Rose, Martin
    Sakai, Shinichi
    Van den Eede, Nele
    van der Veen, Ike
    A novel abbreviation standard for organobromine, organochlorine and organophosphorus flame retardants and some characteristics of the chemicals2012Ingår i: Environment International, ISSN 0160-4120, E-ISSN 1873-6750, Vol. 49, s. 57-82Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    Ever since the interest in organic environmental contaminants first emerged 50 years ago, there has been a need to present discussion of such chemicals and their transformation products using simple abbreviations so as to avoid the repetitive use of long chemical names. As the number of chemicals of concern has increased, the number of abbreviations has also increased dramatically, sometimes resulting in the use of different abbreviations for the same chemical. In this article, we propose abbreviations for flame retardants (FRs) substituted with bromine or chlorine atoms or including a functional group containing phosphorus, i.e. BFRs, CFRs and PFRs, respectively. Due to the large number of halogenated and organophosphorus FRs, it has become increasingly important to develop a strategy for abbreviating the chemical names of FRs. In this paper, a two step procedure is proposed for deriving practical abbreviations (PRABs) for the chemicals discussed. In the first step, structural abbreviations (STABs) are developed using specific STAB criteria based on the FR structure. However, since several of the derived STABs are complicated and long, we propose instead the use of PRABs. These are, commonly, an extract of the most essential part of the STAB, while also considering abbreviations previously used in the literature. We indicate how these can be used to develop an abbreviation that can be generally accepted by scientists and other professionals involved in FR related work. Tables with PRABs and STABs for BFRs, CFRs and PERs are presented, including CAS (Chemical Abstract Service) numbers, notes of abbreviations that have been used previously, CA (Chemical Abstract) name, common names and trade names, as well as some fundamental physicochemical constants.

  • 23.
    Bergman, Åke
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    van den Berg, Martin
    Some bifocal views of risks of BFRs2007Ingår i: Organohalogen Compounds: Plenary Lecture, 2007, s. 7-9Konferensbidrag (Refereegranskat)
  • 24. Birnbaum, Linda S.
    et al.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Brominated and Chlorinated Flame Retardants: The San Antonio Statement2010Ingår i: Journal of Environmental Health Perspectives, ISSN 0091-6765, E-ISSN 1552-9924, Vol. 118, nr 12, s. A514-A515Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    The San Antonio Statement on Brominated and Chlorinated Flame Retardants addresses the growing concern in the scientific community about the persistent, bioaccumulative, and toxic properties of brominated and chlorinated organic flame retardants (BFRs and CFRs, respectively) and the exposure to humans and wildlife as a result of intensive use. Nearly 150 scientists from 22 countries have signed the statement since it was presented at the 30th International Symposium on Halogenated Persistent Organic Pollutants (Dioxin 2010), held 1217 September 2010 in San Antonio, Texas. The scientist signatories are experts on the health effects and environmental fate of BFRs and CFRs and environmental contaminants in general. The International Panel on Chemical Pollution (IPCP), an international network of scientists working on various aspects of chemical pollution, also has approved the statement.

  • 25. Björk, C
    et al.
    Nenonen, H
    Giwercman, Alexander
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Rylander, Lars
    Lundberg Giwercman, Yvonne
    The impact of CB-153 and p,p’-DDE on androgen receptor function2009Ingår i: Organohalogen Compounds, Vol. 71, Peking, 2009, s. 816-819Konferensbidrag (Refereegranskat)
    Ladda ner fulltext (pdf)
    FULLTEXT01
  • 26. Björk, Christel
    et al.
    Nenonen, Hannah
    Giwercman, Aleksander
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Rylander, Lars
    Giwercman, Yvonne Lundberg
    Persistent organic pollutants have dose and CAG repeat length dependent effects on androgen receptor activity in vitro2011Ingår i: Reproductive Toxicology, ISSN 0890-6238, E-ISSN 1873-1708, Vol. 32, s. 293-297Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Recently, the effect of exposure to persistent organic pollutants (POPS) on sperm concentration was only seen in men with a short androgen receptor (AR) gene CAG repeat. In order to investigate whether these effects could be observed also in vitro, we tested the impact of 2,2’,4,4’,5,5’-hexachlorobiphenyl (CB-153) and 1,1-bis-(4-chlorophenyl)-2,2-dichloroethene (4,4’-DDE) on 5 alpha-dihydrotestosterone activated ARs containing 16,22 and 28 CAG repeats, respectively. Single exposure to 4,4’-DDE had the most pronounced effect on the AR activity containing 16 CAG repeats, whereas 28 CAG was the most sensitive variant when a mixture of the two compounds was added. Thus, our in vitro results have confirmed the in vivo data indicating a CAG repeat length dependent effect of endocrine disrupters on the AR activity.

  • 27.
    Bogdanska, Jasna
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Borg, Daniel
    Bergström, Ulrika
    Mellring, Maria
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi.
    DePierre, Joseph
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Nobel, Stefan
    Tissue distribution of C-14-labelled perfluorooctanoic acid in adult mice after 1-5 days of dietary exposure to an experimental dose or a lower dose that resulted in blood levels similar to those detected in exposed humans2020Ingår i: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 239, artikel-id 124755Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Perfluorooctanoic acid (PFOA), a global environmental pollutant detected in both wildlife and human populations, has several pathophysiological effects in experimental animals, including hepatotoxicity, immunotoxicity, and developmental toxicity. However, details concerning the tissue distribution of PFOA, in particular at levels relevant to humans, are lacking, which limits our understanding of how humans, and other mammals, may be affected by this compound. Therefore, we characterized the tissue distribution of C-14-PFOA in mice in the same manner as we earlier examined its analogues perfluorooctanesulfonate (PFOS) and perfluorobutanesulfonate (PFBS) in order to allow direct comparisons. Following dietary exposure of adult male C57/BL6 mice for 1, 3 or 5 days to a low dose (0.06 mg/kg/day) or a higher experimental dose (22 mg/kg/day) of C-14-PFOA, both scintillation counting and whole-body autoradiography revealed the presence of PFOA in most of the 19 different tissues examined, demonstrating its ability to leave the bloodstream and enter tissues. There were no differences in the pattern of tissue distribution with the low and high dose and the tissue-to-blood ratios were similar. At both doses, PFOA levels were highest in the liver, followed by blood, lungs and kidneys. The body compartments estimated to contain the largest amounts of PFOA were the liver, blood, skin and muscle. In comparison with our identical studies on PFOS and PFBS, PFOA reached considerably higher tissue levels than PFBS, but lower than PFOS. Furthermore, the distribution of PFOA differed notably from that of PFOS, with lower tissue-to-blood ratios in the liver, lungs, kidneys and skin.

  • 28.
    Bogdanska, Jasna
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Borg, Daniel
    Sundström, Maria
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Bergström, Ulrika
    Halldin, Krister
    Abedi-Valugerdi, Manuchehr
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Nelson, Buck
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    DePierre, Joseph
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Nobel, Stefan
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Tissue distribution of (35)S-labelled perfluorooctane sulfonate in adult mice after oral exposure to a low environmentally relevant dose or a high experimental dose2011Ingår i: Toxicology, ISSN 0300-483X, E-ISSN 1879-3185, Vol. 284, nr 1-3, s. 54-62Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The widespread environmental pollutant perfluorooctane sulfonate (PFOS), detected in most animal species including the general human population, exerts several effects on experimental animals, e.g., hepatotoxicity, immunotoxicity and developmental toxicity. However, detailed information on the tissue distribution of PFOS in mammals is scarce and, in particular, the lack of available information regarding environmentally relevant exposure levels limits our understanding of how mammals (including humans) may be affected. Accordingly, we characterized the tissue distribution of this compound in mice, an important experimental animal for studying PFOS toxicity. Following dietary exposure of adult male C57/BL6 mice for 1-5 days to an environmentally relevant (0.031 mg/kg/day) or a 750-fold higher experimentally relevant dose (23 mg/kg/day) of (35)S-PFOS, most of the radioactivity administered was recovered in liver, bone (bone marrow), blood, skin and muscle, with the highest levels detected in liver, lung, blood, kidney and bone (bone marrow). Following high daily dose exposure, PFOS exhibited a different distribution profile than with low daily dose exposure, which indicated a shift in distribution from the blood to the tissues with increasing dose. Both scintillation counting (with correction for the blood present in the tissues) and whole-body autoradiography revealed the presence of PFOS in all 19 tissues examined, with identification of thymus as a novel site for localization for PFOS and bone (bone marrow), skin and muscle as significant body compartments for PFOS. These findings demonstrate that PFOS leaves the bloodstream and enters most tissues in a dose-dependent manner.

  • 29.
    Bogdanska, Jasna
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Sundström, Maria
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Bergström, Ulrika
    Borg, Daniel
    Abedi-Valugerdi, Manuchehr
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    DePierre, Joseph
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Nobel, Stefan
    Tissue distribution of S-35-labelled perfluorobutanesulfonic acid in adult mice following dietary exposure for 1-5 days2014Ingår i: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 98, s. 28-36Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Perfluorobutanesulfonyl fluoride (PBSF) has been introduced as a replacement for its eight-carbon homolog perfluorooctanesulfonyl fluoride (POSF) in the manufacturing of fluorochemicals. Fluorochemicals derived from PBSF may give rise to perfluorobutanesulfonic acid (PFBS) as a terminal degradation product. Although basic mammalian toxicokinetic data exist for PFBS, information on its tissue distribution has only been reported in one study focused on rat liver. Therefore, here we characterized the tissue distribution of PFBS in mice in the same manner as we earlier examined its eight-carbon homolog perfluorooctanesulfonate (PFOS) to allow direct comparisons. Following dietary exposure of adult male C57/BL6 mice for 1,3 or 5 d to 16 mg S-35-PFBS kg(-1) d(-1), both scintillation counting and whole-body autoradiography (WBA) revealed the presence of PFBS in all of the 20 different tissues examined, demonstrating its ability to leave the bloodstream and enter tissues. After 5 d of treatment the highest levels were detected in liver, gastrointestinal tract, blood, kidney, cartilage, whole bone, lungs and thyroid gland. WBA revealed relatively high levels of PFBS in male genital organs as well, with the exception of the testis. The tissue levels increased from 1 to 3 d of exposure but appeared thereafter to level-off in most cases. The estimated major body compartments were whole bone, liver, blood, skin and muscle. This exposure to PFBS resulted in 5-40-fold lower tissue levels than did similar exposure to PFOS, as well as in a different pattern of tissue distribution, including lower levels in liver and lungs relative to blood.

  • 30.
    Bogdanska, Jasna
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Sundström, Maria
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Bergström, Ulrika
    Institutionen för miljötoxikologi, Uppsala universitet.
    Borg, Daniel
    Institutet för miljömedicin, Karolinska institutet.
    Abedi-Valugerdi, Mauchehr
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Nelson, Buck
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    DePierre, Joseph
    Institutionen för biokemi och biofysik, Department of Biochemistry and Biophysics.
    Nobel, Stefan
    Department of molecular medicin and surgery, Karolinska institutet.
    Tissue distribution of 35S-labelled perfluorobutane sulfonic acid in adult mice following dietary exposure for 1-5 daysManuskript (preprint) (Övrigt vetenskapligt)
  • 31. Borg, D.
    et al.
    Bogdanska, J.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Sundström, Maria
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    Nobel, S.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Håkansson, H.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    DePierre, J. W.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Halldin, K.
    Bergstrom, U.
    Tissue distribution of S-35-labelled perfluorooctane sulfonate (PFOS) in C57Bl/6 mice following late gestational exposure2010Ingår i: Reproductive Toxicology, ISSN 0890-6238, E-ISSN 1873-1708, Vol. 30, nr 4, s. 558-565Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Exposure of rodents in utero to perfluorooctane sulfonate (PFOS) impairs perinatal development and survival Following intravenous or gavage exposure of C57Bl/6 mouse dams on gestational day (GD) 16 to S-35-PFOS (12 5 mg/kg) we determined the distribution in dams fetuses (GD18 and GD20) and pups (postnatal day 1 PND1) employing whole-body autoradiography and liquid scintillation counting In dams levels were highest in liver and lungs After placental transfer S-35-PFOS was present on GD18 at 2-3 times higher levels in lungs liver and kidneys than in maternal blood In PND1 pups levels in lungs were significantly higher than in GD18 fetuses A heterogeneous distribution of S-35-PFOS was observed in brains of fetuses and pups with levels higher than in maternal brain This first demonstration of substantial localization of PFOS to both perinatal and adult lungs is consistent with evidence describing the lung as a target for the toxicity of PFOS at these ages.

  • 32. Borg, Daniel
    et al.
    Bogdanska,, Jasna
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Sundström, Maria
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Nobel, Stefan
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Håkansson, Helen
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    DePierre, Joseph
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
    Halldin, Krister
    Bergström, Ulrika
    Perinatal tissue distribution of perfluorooctane sulphonate (PFOS) in mice2009Ingår i: Toxicology Letters, ISSN 0378-4274, E-ISSN 1879-3169, Vol. 189, nr SI, s. S147-S147Artikel i tidskrift (Refereegranskat)
  • 33.
    Cantillana, T.
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Lindström, V.
    Eriksson, L.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för fysikalisk kemi, oorganisk kemi och strukturkemi.
    Brandt, I.
    Bergman, Å.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Interindividual differences in o,p '-DDD enantiomer kinetics examined in Göttingen minipigs2009Ingår i: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Chemosphere, Vol. 76, nr 2, s. 167-172Artikel i tidskrift (Refereegranskat)
  • 34.
    Cantillana, Tatiana
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Hermansson, Veronica
    Brandt, Ingvar
    Bergman, Åke
    Enantiomer fractions of o,p'-DDD in plasma and tissues from Göttingen minipigs2007Ingår i: Organohalogen Compounds: Chiral Compounds, 2007, s. 271-274Konferensbidrag (Refereegranskat)
  • 35.
    Cantillana, Tatiana
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Sundström, Maria
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Synthesis of 2-(4-chlorophenyl)-2-(4-chloro-3-thiophenol)-1,1-dichloroethene (3-SH-DDE) via Newman-Kwart rearrangement - A precursor for synthesis of radiolabeled and unlabeled alkylsulfonyl-DDEs2009Ingår i: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, ISSN 0045-6535, Vol. 76, nr 6, s. 805-810Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    For the first time, a pathway for synthesis of 2-(4-chlorophenyl)-2-(4-chloro-3-thiophenol)-1,1-dichloroethene (3-SH-DDE), is presented. The compound is of particular interest as a precursor for synthesis of alkylsulfonyl-DDE containing different alkyl groups to discover structural activity relationships, and to promote synthesis of radiolabeled methylsulfonyl-DDE. 2-Chloro-5-methyl phenol was first methylated and further oxidized to the corresponding benzoic acid. The acid was reduced to the corresponding aldehyde (4-chloro-3-methoxy benzaldehyde) via 4-chloro-3-methoxy-benzene methanol. A lead/aluminium bimetal system was used to carry out the reductive addition of tetrachloromethane to 4-chloro-3-methoxy benzaldehyde to obtain 2,2,2-trichloro-1-(4-chloro-3-methoxyphenyl)ethanol,the desired starting material to synthesize the DDT-analogue (2-(4-chlorophenyl)-2-(4-chloro-3-methoxy-phenyl)-1,1,1-trichloroethane). Elimination of hydrochloric acid and removal of the methyl group led to the 3-OH-DDE. The Newman-Kwart rearrangement was applied to convert 3-OH-DDE to 3-SH-DDE via the dimethyl-carbarnothioate derivative. 3-SH-DDE is then used as a precursor for the radiolabel synthesis. The overall yield to acquire 3-SH-DDE after 11 steps was 3%. The step with the lowest yield was the DDT-analog synthesis with a yield of 30%. All other step had a yield of >50%. 3-SH-DDE was methylated with C-14-labeled iodomethane and oxidized by hydrogen peroxide to obtain 3-[C-14]MeSO2-DDE in an overall yield of 30%.

  • 36. Caporale, Nicolò
    et al.
    Leemans, Michelle
    Birgersson, Lina
    Germain, Pierre-Luc
    Cheroni, Cristina
    Borbély, Gábor
    Engdahl, Elin
    Lindh, Christian
    Bardini Bressan, Raul
    Cavallo, Francesca
    Chorev, Nadav Even
    D'Agostino, Giuseppe Alessandro
    Pollard, Steven M.
    Rigoli, Marco Tullio
    Tenderini, Erika
    Lopez Tobon, Alejandro
    Trattaro, Sebastiano
    Troglio, Flavia
    Zanella, Matteo
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap. Swedish Toxicology Sciences Research Center, Sweden; Örebro University, Sweden.
    Damdimopoulou, Pauliina
    Jönsson, Maria
    Kiess, Wieland
    Kitraki, Efthymia
    Kiviranta, Hannu
    Nånberg, Eewa
    Öberg, Mattias
    Rantakokko, Panu
    Rudén, Christina
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap.
    Söder, Olle
    Bornehag, Carl-Gustaf
    Demeneix, Barbara
    Fini, Jean-Baptiste
    Gennings, Chris
    Rüegg, Joëlle
    Sturve, Joachim
    Testa, Giuseppe
    From cohorts to molecules: Adverse impacts of endocrine disrupting mixtures2022Ingår i: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 375, nr 6582Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    INTRODUCTION: Endocrine disrupting chemicals (EDCs) are compounds that interfere with physiological hormonal regulation. Humans are pervasively exposed to many different EDCs, and a growing body of evidence indicates that early life exposure to such EDC mixtures can induce changes in the human organism that underlie increased susceptibility to diseases throughout the life span, including neurodevelopmental disorders. Chemical regulation is, however, entirely based on the risk assessment of individual compounds, leaving the real-life impact of chemical mixtures unexamined and unregulated. This is relevant insofar as cumulative exposure to multiple compounds may be associated with adverse health outcomes even when the concentrations of individual chemicals fall below the regulatory dose.

    RATIONALE: We set out to make the epidemiological associations between exposure to mixtures and health outcomes experimentally tractable, defining molecular pathways and dose responses that could be translated back to actual human exposures and thereby refine current risk assessment practices. As opposed to previous studies that focused on single compounds, we identified and tested an EDC mixture associated with adverse neurodevelopmental outcomes in the Swedish Environmental Longitudinal, Mother and child, Asthma and allergy (SELMA) pregnancy cohort (MIX N) by integrating epidemiological data with experimental toxicology and characterized real life–relevant exposure.

    RESULTS: We used weighted quantile sum (WQS) regression to identify chemicals associated with language delay in children and included those chemicals in MIX N. MIX N was synthesized following the relative proportions and total concentrations found in the SELMA cohort. It was then tested in both in vitro and in vivo models. In human fetal primary neural stem cells and three-dimensional cortical brain organoids differentiated from human pluripotent stem cells, transcriptomic analysis showed that MIX N interferes with hormonal pathways and dysregulates expression of genes and biological pathways that are causally linked to autism spectrum disorders. Data from experiments in Xenopus leavis and Danio rerio, in vivo models validated by the Organisation for Economic Co-operation and Development (OECD), confirmed thyroid function as one of the key and unifying points of vulnerability to MIX N and linked thyroid disruption to neurodevelopmental effects measured as alterations in locomotor activity. The resulting dose-response relationships were then used to estimate a point of departure (POD), which is the toxicological measure to estimate no-effect concentration. This enabled us to apply a similar mixture approach (SMACH) where we (i) identified individuals in the SELMA study who were sufficiently similarly exposed compared with the experimental mixtures and (ii) determined the proportion of the SELMA children with exposure ranges of concern using the POD as reference.

    CONCLUSION: Integrating experimental and epidemiological evidence, we established mechanistic and correlative evidence for neurodevelopmental adversities of an EDC mixture associated with language delay. Using the generated experimental data in a risk assessment concept, we found increased odds of language delay in offspring of up to 54% of pregnant women. These results emphasize the need to take mixtures into account during chemical testing and risk assessment and provide an integrative framework to guide risk assessment strategies.

     

  • 37.
    Christiansson, Anna
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Eriksson, Johan
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Teclechiel, Daniel
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Identification and quantification of products formed via photolysis of decabromodiphenyl ether2009Ingår i: Environmental Science and Pollution Research, ISSN 0944-1344, E-ISSN 1614-7499, Vol. 16, nr 3, s. 312-321Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    BACKGROUND, AIM, AND SCOPE: Decabromodiphenyl ether (DecaBDE) is used as an additive flame retardant in polymers. It has become a ubiquitous environmental contaminant, particularly abundant in abiotic media, such as sediments, air, and dust, and also present in wildlife and in humans. The main DecaBDE constituent, perbrominated diphenyl ether (BDE-209), is susceptible to transformations as observed in experimental work. This work is aimed at identifying and assessing the relative amounts of products formed after UV irradiation of BDE-209. MATERIALS AND METHODS: BDE-209, dissolved in tetrahydrofuran (THF), methanol, or combinations of methanol/water, was exposed to UV light for 100 or 200 min. Samples were analyzed by gas chromatography/mass spectrometry (electron ionization) for polybrominated diphenyl ethers (PBDEs), dibenzofurans (PBDFs), methoxylated PBDEs, and phenolic PBDE products. RESULTS: The products formed were hexaBDEs to nonaBDEs, monoBDFs to pentaBDFs, and methoxylated tetraBDFs to pentaBDFs. The products found in the fraction containing halogenated phenols were assigned to be pentabromophenol, dihydroxytetrabromobenzene, dihydroxydibromodibenzofuran, dihydroxytribromodibenzofuran, and dihydroxytetrabromodibenzofuran. The PBDEs accounted for approximately 90% of the total amount of substances in each sample and the PBDFs for about 10%. DISCUSSION: BDE-209 is a source of PBDEs primarily present in OctaBDEs but also to some extent in PentaBDEs, both being commercial products now banned within the EU and in several states within the USA. It is notable that OH-PBDFs have not been identified or indicated in any of the photolysis studies performed to date. Formation of OH-PBDFs, however, may occur as pure radical reactions in the atmosphere. CONCLUSIONS: Photolysis of decaBDE yields a wide span of products, from nonaBDEs to hydroxylated bromobenzenes. It is evident that irradiation of decaBDE in water and methanol yields OH-PBDFs and MeO-PBDFs, respectively. BDE-202 (2,2',3,3',5,5',6,6'-octabromodiphenyl ether) is identified as a marker of BDE-209 photolysis. RECOMMENDATIONS AND PERSPECTIVES: BDE-209, the main constituent of DecaBDE, is primarily forming debrominated diphenyl ethers with higher persistence which are more bioaccumulative than the starting material when subjected to UV light. Hence, DecaBDE should be considered as a source of these PBDE congeners in the environment

  • 38.
    Christiansson, Anna
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Hovander, Lotta
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Athanassiadis, Ioannis
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Jakobsson, Kristina
    Department of Occupational and Environmental Medicine, Lund University Hospital.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Polybrominated diphenyl ethers in aircraft cabins – a source of human exposure?2008Ingår i: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 73, nr 10, s. 1654-1660Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Commercial aircrafts need a high degree of fire protection for passenger safety. Brominated flame retardants (BFRs), including polybrominated diphenyl ethers (PBDEs), may be used for this purpose. Because PBDEs readily absorb to dust particles, aircraft crew and passengers may receive significant PBDEs exposure via inhalation. The aims of this work were to assess whether PBDEs could be found in aircraft cabin dust and whether serum levels of PBDEs increased in passengers after long-distance flights. Hence nine subjects on intercontinental flights collected cabin dust samples, as well as donated blood samples before departure and after return to Sweden. Two subjects who were domestic frequent flyers were also investigated. The levels of PBDEs in dust and serum were determined by GC/MS in electron capture negative ionization (ECNI) mode. Authentic reference substances were used for identification and quantitation. PBDEs were found in all aircraft dust samples at high concentrations, higher than in common household dust. Congener patterns indicated that the technical products PentaBDE, OctaBDE and DecaBDE were used in the aircrafts. Serum concentrations in the travellers were similar to those observed in Swedish residents in general. Post-travel serum levels of BDE-28, BDE-99, BDE-100, BDE-153, and BDE-154 were significantly higher (p < 0.05) than concentrations prior to travel. The findings from this pilot study call for investigations of occupational exposures to PBDEs in cabin and cockpit crews.

  • 39.
    Dahlberg, Anna-Karin
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi.
    Lindberg Chen, Vivian
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi.
    Larsson, Kjell
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi. Swedish Toxicology Sciences Research Center (Swetox), Sweden.
    Asplund, Lillemor
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap och analytisk kemi.
    Hydroxylated and methoxylated polybrominated diphenyl ethers in long-tailed ducks (Clangula hyemalis) and their main food, Baltic blue mussels (Mytilus trossulus x Mytilus edulis).2016Ingår i: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 144, s. 1475-1483Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Long-tailed ducks (Clangula hyemalis) that breed in northern Europe and western Siberia and commonly winter in the Baltic Sea, are threatened by a significant population decrease. The ducks are, by primarily feeding on Baltic blue mussels (Mytilus trossulus x Mytilus edulis) while wintering in the Baltic Sea, potentially subjected to high levels of toxic hydroxylated polybrominated diphenyl ethers (OH-PBDEs). To assess long-tailed ducks exposure to polybrominated phenols (PBPs), polybrominated anisoles (PBAs), hydroxylated polybrominated diphenyl ethers (OH-PBDEs), their methylated counterparts (MeO-PBDEs) and polybrominated diphenyl ethers (PBDEs), livers of ten long-tailed ducks wintering in the Baltic Sea were analysed. Pattern and levels of analytes in long-tailed ducks (liver) and blue mussels sampled in March and May at nine sites in the Baltic Sea were compared. The geometric mean concentration (ng/g l.w.) in livers of long-tailed ducks and Baltic blue mussels were: Sigma(2)PBPs: 0.57 and 48; Sigma 2PBAs: 0.83 and 11; Sigma 7OH-PBDEs: 6.1 and 45; Sigma 7MeO-PBDEs: 3.8 and 69; Sigma 7PBDEs: 8.0 and 7.2, respectively. Based on an estimated daily intake of 450 g fresh blue mussel meat, long-tailed ducks daily dietary intake of brominated substances while foraging in the Baltic Sea in March-May was estimated to; 390 ng Sigma(2)PBPs, 90 ng Sigma 2PBAs, 370 ng Sigma 7OH-PBDEs, 590 ng Sigma 7MeO-PBDEs and 59 ng Sigma 7PBDEs. The low levels of PBPs, PBAs, OH-PBDEs and MeO-PBDEs in the long-tailed duck livers compared to blue mussel, despite a continuous daily intake, suggest that these compounds are poorly retained in long-tailed ducks.

  • 40.
    Dahlberg, Anna-Karin
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Norrgran, Jessica
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Hovander, Lotta
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Asplund, Lillemor
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Recovery discrepancies of OH-PBDEs and polybromophenols in human plasma and cat serum versus herring and long-tailed duck plasma2014Ingår i: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 94, s. 97-103Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) have been identified as metabolites of polybrominated diphenyl ethers (PBDEs) and/or as natural products. The OH-PBDEs and polybromophenols have come into focus over the last decade due to their abundance in biota and their potential adverse health effects. The present recovery study aims to validate a commonly used method (published by Hovander et al. 2000) for OH-PBDE analysis in human plasma. Further, the authors intended to determine the method's applicability to serum/plasma matrices from other species than humans. The investigated matrices were human plasma, cat serum, herring- and long-tailed duck plasma. The recovery study included nine OH-PBDEs, four polybromophenols and three methoxylated PBDEs (MeO-PBDEs). Five replicates of each matrix were spiked with these compounds at two dose levels; a low dose (0.5 ng) and a high dose (5 ng) and were cleaned up according to the Hovander method. The recovery of OH-PBDEs and polybromophenols in human plasma and cat serum were high and reproducible at both dose levels whereas the recovery for herring and long-tailed duck plasma were low and insufficient with great variability amongst OH-PBDE congeners at both dose levels. Our data show that the method can be fully applied to matrices like human plasma and cat serum but not for herring and long-tailed duck plasma without further method development. Hence care needs to be taken when applying the method onto other blood matrices without validation since the present study have demonstrated that the recoveries may differ amongst OH-PBDE congeners and specie.

  • 41. Dai, Qingyuan
    et al.
    Xu, Xijin
    Eskenazi, Brenda
    Asante, Kwadwo Ansong
    Chen, Aimin
    Fobil, Julius
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap. Örebro University, Sweden; Tongji University, China.
    Brennan, Lesley
    Sly, Peter D.
    Nnorom, Innocent Chidi
    Pascale, Antonio
    Wang, Qihua
    Zeng, Eddy Y.
    Zeng, Zhijun
    Landrigan, Philip J.
    Drisse, Marie-Noel Brune
    Huo, Xia
    Severe dioxin-like compound (DLC) contamination in e-waste recycling areas: An under-recognized threat to local health2020Ingår i: Environment International, ISSN 0160-4120, E-ISSN 1873-6750, Vol. 139, artikel-id 105731Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Electrical and electronic waste (e-waste) burning and recycling activities have become one of the main emission sources of dioxin-like compounds (DLCs). Workers involved in e-waste recycling operations and residents living near e-waste recycling sites (EWRS) are exposed to high levels of DLCs. Epidemiological and experimental in vivo studies have reported a range of interconnected responses in multiple systems with DLC exposure. However, due to the compositional complexity of DLCs and difficulties in assessing mixture effects of the complex mixture of e-waste-related contaminants, there are few studies concerning human health outcomes related to DLC exposure at informal EWRS. In this paper, we have reviewed the environmental levels and body burdens of DLCs at EWRS and compared them with the levels reported to be associated with observable adverse effects to assess the health risks of DLC exposure at EWRS. In general, DLC concentrations at EWRS of many countries have been decreasing in recent years due to stricter regulations on e-waste recycling activities, but the contamination status is still severe. Comparison with available data from industrial sites and well-known highly DLC contaminated areas shows that high levels of DLCs derived from crude e-waste recycling processes lead to elevated body burdens. The DLC levels in human blood and breast milk at EWRS are higher than those reported in some epidemiological studies that are related to various health impacts. The estimated total daily intakes of DLCs for people in EWRS far exceed the WHO recommended total daily intake limit. It can be inferred that people living in EWRS with high DLC contamination have higher health risks. Therefore, more well-designed epidemiological studies are urgently needed to focus on the health effects of DLC pollution in EWRS. Continuous monitoring of the temporal trends of DLC levels in EWRS after actions is of highest importance.

  • 42. Darnerud, Per Ola
    et al.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljövetenskap. Örebro University, Sweden; Tongji University, China.
    Critical review on disposition of chlorinated paraffins in animals and humans2022Ingår i: Environment International, ISSN 0160-4120, E-ISSN 1873-6750, Environment International, ISSN 0160-4120, Vol. 163, artikel-id 107195Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    Even though the chlorinated paraffins (CPs) have been on the environmental pollution agenda throughout the last 50 years it is a class of chemicals that only now is discussed in terms of an emerging issue with extensive annual publication rates. Major reviews on CPs have been produced, but a deeper understanding of the chemical fate of CPs, including formation of metabolites in animals and humans, is still missing. Thus, the present review aims to critically compile our present knowledge on the disposition, i.e. Adsorption, Disposition, Metabolism, and Excretion (ADME) of CPs in biota and to identify research needs.

    We conclude that CPs could be effectively absorbed from the gastro-intestinal tract (GI) tract, and probably also from the lungs, and transported to various organs. A biphasic elimination is suggested, with a rapid initial phase followed by a terminal phase, the latter (e.g., fat tissues) covering half-lives of weeks and months. CPs are metabolized in the liver and excreted mainly via the bile and faeces, and the metabolic rate and type of metabolites are dependent on chlorine content and chain length. Results that strengthen CP metabolism are in vivo findings of phase II metabolites in bile, and CP degradation to carbon fragments in experimental animals. Still the metabolic transformations of CPs are poorly studied, and no metabolic scheme has yet been presented. Further, toxicokinetic mass balance calculations suggest that a large part of a given dose (not found as parent compound) is transformation products of CPs, and in vitro metabolism studies present numerous CP metabolites (e.g., chloroalkenes, chlorinated ketones, aldehydes, and carboxylic acids).

  • 43. DiGangi, Joseph
    et al.
    Blum, Arlene
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    de Wit, Cynthia A.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för tillämpad miljövetenskap (ITM).
    Lucas, Donald
    Mortimer, David
    Schecter, Arnold
    Scheringer, Martin
    Shaw, Susan
    Webster, Tomas F.
    San Antonio Statement on brominated and chlorinated flame retardants2010Ingår i: Environmental Health Perspectives, ISSN 0091-6765, Vol. 118, nr 12, s. A516-A518Artikel i tidskrift (Refereegranskat)
  • 44. Dingemans, Milou M L
    et al.
    de Groot, Aart
    van Kleef, Regina G D M
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    van den Berg, Martin
    Vijverberg, Henk P M
    Westerink, Remco H S
    Hydroxylation increases the neurotoxic potential of BDE-47 to affect exocytosis and calcium homeostasis in PC12 cells.2008Ingår i: Journal of Environmental Health Perspectives, ISSN 0091-6765, E-ISSN 1552-9924, Vol. 116, nr 5, s. 637-43Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    BACKGROUND: Oxidative metabolism, resulting in the formation of hydroxylated polybrominated diphenyl ether (PBDE) metabolites, may enhance the neurotoxic potential of brominated flame retardants. OBJECTIVE: Our objective was to investigate the effects of a hydroxylated metabolite of 2,2',4,4'-tetra-bromodiphenyl ether (BDE-47; 6-OH-BDE-47) on changes in the intracellular Ca2+ concentration ([Ca2+]i) and vesicular catecholamine release in PC12 cells. METHODS: We measured vesicular catecholamine release and [Ca2+]i using amperometry and imaging of the fluorescent Ca2+-sensitive dye Fura-2, respectively. RESULTS: Acute exposure of PC12 cells to 6-OH-BDE-47 (5 microM) induced vesicular catecholamine release. Catecholamine release coincided with a transient increase in [Ca2+]i, which was observed shortly after the onset of exposure to 6-OH-BDE-47 (120 microM). An additional late increase in [Ca2+]i was often observed at &gt; or =1 microM 6-OH-BDE-47. The initial transient increase was absent in cells exposed to the parent compound BDE-47, whereas the late increase was observed only at 20 microM. Using the mitochondrial uncoupler carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) and thapsigargin to empty intracellular Ca2+ stores, we found that the initial increase originates from emptying of the endoplasmic reticulum and consequent influx of extracellular Ca2+, whereas the late increase originates primarily from mitochondria. CONCLUSION: The hydroxylated metabolite 6-OH-BDE-47 is more potent in disturbing Ca2+ homeostasis and neurotransmitter release than the parent compound BDE-47. The present findings indicate that bioactivation by oxidative metabolism adds considerably to the neurotoxic potential of PBDEs. Additionally, based on the observed mechanism of action, a cumulative neurotoxic effect of PBDEs and ortho-substituted polychlorinated biphenyls on [Ca2+]i cannot be ruled out.

  • 45. Dingemans, Milou M. L.
    et al.
    Heusinkveld, Harm J.
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    van den Berg, Martin
    Westerink, Remco H. S.
    Bromination Pattern of Hydroxylated Metabolites of BDE-47 Affects Their Potency to Release Calcium from Intracellular Stores in PC12 Cells2010Ingår i: Journal of Environmental Health Perspectives, ISSN 0091-6765, E-ISSN 1552-9924, Vol. 118, nr 4, s. 519-525Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    BACKGROUND: Brominated flame retardants, including the widely used polybrominated diphenyl ethers (PBDEs), have been detected in humans, raising concern about possible neurotoxicity. Recent research demonstrated that the hydroxylated metabolite 6-OH-BDE-47 increases neurotransmitter release by releasing calcium ions (Ca2+) from intracellular stores at much lower concentrations than its environmentally relevant parent congener BDE-47. Recently, several other hydroxylated BDE-47 metabolites, besides 6-OH-BDE-47, have been detected in human serum and cord blood. OBJECTIVE AND METHODS: To investigate the neurotoxic potential of other environmentally relevant PBDEs and their metabolites, we examined and compared the acute effects of BDE-47, BDE-49, BDE-99, BDE-100, BDE-153, and several metabolites of BDE-47-6-OH-BDE-47 (and its methoxylated analog 6-MeO-BDE-47), 6'-OH-BDE-49, 5-OH-BDE-47, 3-OH-BDE-47, and 4'-OH-BDE-49 on intracellular Ca2+ concentration ([Ca2+](i)), measured using the Ca2+-responsive dye Fura-2 in neuroendocrine pheochromocytoma (PC12) cells. RESULTS: In contrast to the parent PBDEs and 6-MeO-BDE-47, all hydroxylated metabolites induced Ca2+ release from intracellular stores, although with different lowest observed effect concentrations (LOECs). The major intracellular Ca2+ sources were either endoplasmic reticulum (ER; 5-OH-BDE-47 and 6'-OH-BDE-49) or both ER and mitochondria (6-OH-BDE-47, 3-OH-BDE-47, and 4'-OH-BDE-49). When investigating fluctuations in [Ca2+](i), which is a more subtle end point, we observed lower LOECs for 6-OH-BDE-47 and 4'-OH-BDE-49, as well as for BDE-47. CONCLUSIONS: The present findings demonstrate that hydroxylated metabolites of BDE-47 cause disturbance of the [Ca2+](i). Importantly, shielding of the OH group on both sides with bromine atoms and/or the ether bond to the other phenyl ring lowers the potency of hydroxylated PBDE metabolites.

  • 46. Dingemans, Milou M L
    et al.
    Heusinkveld, Harm J
    de Groot, Aart
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    van den Berg, Martin
    Westerink, Remco H S
    Hexabromocyclododecane (HBCD) inhibits depolarization-induced increase in intracellular calcium levels and neurotransmitter release in PC12 cells.2009Ingår i: Toxicol Sci, ISSN 1096-0929, Vol. 107, nr 2, s. 490-498Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    Environmental levels of the brominated flame retardant (BFR) hexabromocyclododecane (HBCD) have been increasing. HBCD has been shown to cause adverse effects on learning and behavior in mice, as well as on dopamine uptake in rat synaptosomes and synaptic vesicles. For other BFRs, alterations in the intracellular Ca(2+) homeostasis have been observed. Therefore, the aim of this study was to investigate whether the technical HBCD mixture and individual stereoisomers affect the intracellular Ca(2+) concentration ([Ca(2+)](i)) in a neuroendocrine in vitro model (PC12 cells). [Ca(2+)](i) and vesicular catecholamine release were measured using respectively single-cell Fura-2 imaging and amperometry. Exposure of PC12 cells to the technical HBCD mixture or individual stereoisomers did neither affect basal [Ca(2+)](i), nor the frequency of basal neurotransmitter release. However, exposure to HBCD (0 - 20 muM) did cause a dose-dependent reduction of a subsequent depolarization-evoked increase in [Ca(2+)](i). This effect was apparent only when HBCD was applied at least 5 min before depolarization (maximum effect after 20 min exposure). The effects of alpha- and beta-HBCD were comparable to that of the technical mixture, whereas the inhibitory effect of gamma-HBCD was larger. Using specific blockers of L-, N- or P/Q-type voltage-gated Ca(2+) channels (VGCCs) it was shown that the inhibitory effect of HBCD is not VGCC-specific. Additionally, the number of cells showing depolarization-evoked neurotransmitter release was markedly reduced following HBCD exposure. Summarizing, HBCD inhibits depolarization-evoked [Ca(2+)](i) and neurotransmitter release. As increasing HBCD levels should be anticipated, these findings justify additional efforts to establish an adequate exposure, hazard and risk assessment.

  • 47. Dingemans, Milou M. L.
    et al.
    Heusinkveld, Harm J.
    de Groot, Aart
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK), Avdelningen för miljökemi.
    van den Berg, Martin
    Westerink, Remco H. S.
    Hexabromocyclododecane Inhibits Depolarization-Induced Increase in Intracellular Calcium Levels and Neurotransmitter Release in PC12 Cells2009Ingår i: Toxicological Sciences, ISSN 1096-6080, E-ISSN 1096-0929, Vol. 107, nr 2, s. 490-497Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Environmental levels of the brominated flame retardant (BFR) hexabromocyclododecane (HBCD) have been increasing. HBCD has been shown to cause adverse effects on learning and behavior in mice, as well as on dopamine uptake in rat synaptosomes and synaptic vesicles. For other BFRs, alterations in the intracellular Ca2+ homeostasis have been observed. Therefore, the aim of this study was to investigate whether the technical HBCD mixture and individual stereoisomers affect the intracellular Ca2+ concentration ([Ca2+](i)) in a neuroendocrine in vitro model (PC12 cells). [Ca2+](i) and vesicular catecholamine release were measured using respectively single-cell Fura-2 imaging and amperometry. Exposure of PC12 cells to the technical HBCD mixture or individual stereoisomers did neither affect basal [Ca2+](i), nor the frequency of basal neurotransmitter release. However, exposure to HBCD (0-20 mu M) did cause a dose-dependent reduction of a subsequent depolarization-evoked increase in [Ca2+](i). This effect was apparent only when HBCD was applied at least 5 min before depolarization (maximum effect after 20 min exposure). The effects of alpha- and beta-HBCD were comparable to that of the technical mixture, whereas the inhibitory effect of gamma-HBCD was larger. Using specific blockers of L-, N- or P/Q-type voltage-gated Ca2+ channels (VGCCs) it was shown that the inhibitory effect of HBCD is not VGCC-specific. Additionally, the number of cells showing depolarization-evoked neurotransmitter release was markedly reduced following HBCD exposure. Summarizing, HBCD inhibits depolarization-evoked [Ca2+](i) and neurotransmitter release. As increasing HBCD levels should be anticipated, these findings justify additional efforts to establish an adequate exposure, hazard and risk assessment.

  • 48. Dingemans, Milou M L
    et al.
    Ramakers, Geert M J
    Gardoni, Fabrizio
    van Kleef, Regina G D M
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för miljökemi.
    Di Luca, Monica
    van den Berg, Martin
    Westerink, Remco H S
    Vijverberg, Henk P M
    Neonatal exposure to brominated flame retardant BDE-47 reduces long-term potentiation and postsynaptic protein levels in mouse hippocampus.2007Ingår i: Environ Health Perspect, ISSN 0091-6765, Vol. 115, nr 6, s. 865-70Artikel i tidskrift (Refereegranskat)
  • 49. Dingemans, Milou M. L.
    et al.
    van den Berg, Martin
    Bergman, Åke
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för material- och miljökemi (MMK).
    Westerink, Remco H. S.
    Calcium-Related Processes Involved in the Inhibition of Depolarization-Evoked Calcium Increase by Hydroxylated PBDEs in PC12 Cells2010Ingår i: Toxicological Sciences, ISSN 1096-6080, E-ISSN 1096-0929, Vol. 114, nr 2, s. 302-309Artikel i tidskrift (Refereegranskat)
    Abstract [en]