Since the discovery of cable bacteria more than a decade ago, these multicellular, filamentous sulfur-oxidizing bacteria have been found in a range of sedimentary environments. However, their abundance, diversity, and activity in continental margin sediments overlain by oxygen-deficient waters at water depths of > 100 m remain poorly known. Here we address this by studying five basins along the coasts of California and Mexico. All sediments are organic carbon rich (2.5 wt %–7.5 wt %) and characterized by active iron and sulfur cycling. Nitrate is present in the bottom water at all sites. Results of fluorescence in situ hybridization (FISH) indicate a low areal abundance of cable bacteria (0.2 to 19 m cm−2) in sediments of the hypoxic San Clemente, Catalina, and San Pedro basins and the anoxic San Blas basin. In the anoxic Soledad basin, in contrast, we found abundant cable bacteria near the sediment surface (129 m cm−2). DNA amplicon sequencing detected cable bacteria reads in sediments of the hypoxic San Pedro basin and the anoxic Soledad and San Blas basins. Phylogenetic analysis indicated that the diversity of the amplicon sequence variants (ASVs) was spread across the Candidatus Electrothrix lineage, including multiple ASVs closely related to Electrothrix gigas, a recently discovered species of giant cable bacteria. Additionally, multiple sequences retrieved from the Soledad and San Blas basins revealed affiliation with a clade sister to Electrothrix, hypothesized as a novel genus, suggesting possible relic or novel adaptations of cable bacteria to these anoxic and nitrogenous environments. The areal abundance of cable bacteria was negatively related to sediment Fe / S ratios, suggesting a control by sulfide availability. However, free sulfide in the porewater was only detected at the anoxic Soledad site. Micro-profiling of pH and electric potential points toward a lack of cable bacteria activity at the time of sampling, possibly due to a limitation by a suitable electron donor and/or acceptor. Periodically enhanced organic matter input and associated sulfate reduction and/or inflows of oxic water could alleviate the deficiency, creating the observed niche for diverse cable bacteria.

Coastal areas are an important source of methane (CH4). However, the exact origins of CH4 in the surface waters of coastal regions, which in turn drive sea-air emissions, remain uncertain. To gain a comprehensive understanding of the current and future climate change feedbacks, it is crucial to identify these CH4 sources and processes that regulate its formation and oxidation. This study investigated coastal CH4 dynamics by comparing water column data from six stations located in the brackish Tvärminne Archipelago, Baltic Sea. The sediment biogeochemistry and microbiology were further investigated at two stations (i.e., nearshore and offshore). These stations differed in terms of stratification, bottom water redox conditions, and organic matter loading. At the nearshore station, CH4 diffusion from the sediment into the water column was negligible, because nearly all CH4 was oxidized within the upper sediment column before reaching the sediment surface. On the other hand, at the offshore station, there was significant benthic diffusion of CH4, albeit the majority underwent oxidation before reaching the sediment-water interface, due to shoaling of the sulfate methane transition zone (SMTZ). The potential contribution of CH4 production in the water column was evaluated and was found to be negligible. After examining the isotopic signatures of δ13C-CH4 across the sediment and water column, it became apparent that the surface water δ13C-CH4 values observed in areas with thermal stratification could not be explained by diffusion, advective fluxes, nor production in the water column. In fact, these values bore a remarkable resemblance to those detected below the SMTZ. This supports the hypothesis that the source of CH4 in surface waters is more likely to originate from ebullition than diffusion in stratified brackish coastal systems.

Internal phosphorus (P) loading is widely recognized as a major cause of lake eutrophication. One conventional paradigm states that the magnitude of internal loading through P diffusion is constrained by the presence of iron (Fe) oxides in surface sediments under oxic conditions near the sediment-water interface (SWI). However, biogeochemical P dynamics in Fe-rich sedimentary systems are still not fully understood, especially in eutrophic lakes where intensively coupled organic matter (OM) remineralization and reductive dissolution of Fe-bound P (Fe-P) exist concurrently. Here, we assess the diagenetic processes that govern sedimentary P cycling in two eutrophic Fe-rich lakes in southern Finland, Lake Hiidenvesi and Lake Kytäjärvi, using a combination of porewater and solid-phase analyses. Coupled reductive dissolution of Fe-P and OM remineralization controlled P regeneration in both lakes, with Fe-P acting as the dominant source for porewater P. Vivianite formation likely immobilized sedimentary P in the deepest basin of Hiidenvesi. Elevated P diffusion rates were observed at shallow sites under oxic bottom water conditions in summer in both lakes, stimulated by enhanced remineralization of both freshly- (mostly phytoplankton-origin) and earlier-deposited OM under elevated temperatures. Areas overlain by oxic bottom water contributed more benthic P fluxes to the water column compared to anoxic/hypoxic areas in both lakes during all sampling seasons. Our study suggests that in shallow eutrophic settings with high OM deposition and elevated temperatures, remineralization in upper sediments regenerates P efficiently enough to support a significant amount of P release to the water column even under sedimentary molar Fe/P ratios >20. We also discuss the implication of our findings for lake restoration strategies.

Methane (CH4) cycling in the Baltic Sea is studied through model simulations that incorporate the stable isotopes of CH4 (12C-CH4 and 13C-CH4) in a physical-biogeochemical model. A major uncertainty is that spatial and temporal variations in the sediment source are not well known. Furthermore, the coarse spatial resolution prevents the model from resolving shallow-water near-shore areas for which measurements indicate occurrences of considerably higher CH4 concentrations and emissions compared with the open Baltic Sea. A preliminary CH4 budget for the central Baltic Sea (the Baltic Proper) identifies benthic release as the dominant CH4 source, which is largely balanced by oxidation in the water column and to a smaller degree by outgassing. The contributions from river loads and lateral exchange with adjacent areas are of marginal importance. Simulated total CH4 emissions from the Baltic Proper correspond to an average ∼1/41.5 mmol CH4 m-2 yr-1, which can be compared to a fitted sediment source of ∼1/418 mmol CH4 m-2 yr-1. A large-scale approach is used in this study, but the parameterizations and parameters presented here could also be implemented in models of near-shore areas where CH4 concentrations and fluxes are typically substantially larger and more variable. Currently, it is not known how important local shallow-water CH4 hotspots are compared with the open water outgassing in the Baltic Sea.

Using a combination of sediment trap experiments, sedimentary biogeochemical analyses and mass balance calculations, we conducted a comprehensive quantitative evaluation of the in-lake phosphorus (P) cycles including in both the water and sediment phases for Lake Hiidenvesi, a dimictic eutrophic lake in southern Finland. We explicitly demonstrated the heterogeneity of the in-lake P cycles between basins with distinct morphological features. Enhanced interactions between waters and sediments occur in shallow and non-stratified areas, as evidenced by the magnitudes of gross sedimentation and total internal P loading. In such shallow areas, sediment resuspension contributes over 60% of the total internal P loading throughout the entire open water season. In contrast, sedimentary P cycling is less intensive in deep and stratified areas, where diffusive fluxes account for an average of 70% of total internal P loading. We show that sedimentary P burial plays a key role in controlling the in-lake P cycle. Permanent burial of P showing higher rates and efficiencies tends to occur in deeper areas. Overall, sediments in Lake Hiidenvesi act as a net P sink under modern biogeochemical settings; the lake is in the process of long-term recovery from eutrophication due to the larger annual P output than external loading.

Coastal zones account for 75% of marine methane emissions, despite covering only 15% of the ocean surface area. In these ecosystems, the tight balance between methane production and oxidation in sediments prevents most methane from escaping into seawater. However, anthropogenic activities could disrupt this balance, leading to an increased methane escape from coastal sediments. To quantify and unravel potential mechanisms underlying this disruption, we used a suite of biogeochemical and microbiological analyses to investigate the impact of anthropogenically induced redox shifts on methane cycling in sediments from three sites with contrasting bottom water redox conditions (oxic-hypoxic-euxinic) in the eutrophic Stockholm Archipelago. Our results indicate that the methane production potential increased under hypoxia and euxinia, while anaerobic oxidation of methane was disrupted under euxinia. Experimental, genomic, and biogeochemical data suggest that the virtual disappearance of methane-oxidizing archaea at the euxinic site occurred due to sulfide toxicity. This could explain a near 7-fold increase in the extent of escape of benthic methane at the euxinic site relative to the hypoxic one. In conclusion, these insights reveal how the development of euxinia could disrupt the coastal methane biofilter, potentially leading to increased methane emissions from coastal zones.

Previous studies have demonstrated that e-SO_x can regulate the sedimentary release of phosphorus (P) in brackish and marine sediments. When e-SO_x is active, an iron (Fe) and manganese (Mn) oxide rich layer is formed near the sediment surface, which prevents P release. When e-SO_x becomes inactive, the metal oxide layer is reduced via sulfide-mediated dissolution, and P is subsequently released to the water column. Cable bacteria have been shown to also occur in freshwater sediments. In these sediments, sulfide production is limited, and the metal oxide layer would thus dissolve less efficiently, leaving the P trapped at the sediment surface. This lack of an efficient dissolution mechanism implies that e-SO_x could play an important role in the regulation of P availability in eutrophied freshwater streams. To test this hypothesis, we incubated sediments from a eutrophic freshwater river to investigate the impact of cable bacteria on sedimentary cycling of Fe, Mn and P. High-resolution depth profiling of pH, O₂ and ΣH₂S complemented with FISH analysis and high-throughput gene sequencing showed that the development of e-SO_x activity was closely linked to the enrichment of cable bacteria in incubated sediments. Cable bacteria activity caused a strong acidification in the suboxic zone, leading to the dissolution of Fe and Mn minerals and consequently a strong release of dissolved Fe²⁺ and Mn²⁺ to the porewater. Oxidation of these mobilized ions at the sediment surface led to the formation of a metal oxide layer that trapped dissolved P, as shown by the enrichment of P-bearing metal oxides in the top layer of the sediment and low phosphate in the pore and overlying water. After e-SO_x activity declined, the metal oxide layer did not dissolve and P remained trapped at the surface. Overall, our results suggested cable bacteria can play an important role to counteract eutrophication in freshwater systems.

Recycling of phosphorus (P) from deoxygenated sediments perpetuates eutrophic conditions in parts of the Baltic Sea. Sedimentary organic P is a major source of dissolved P to the water column, but also a sink for permanent P burial. The mechanisms behind these two pathways are, however, largely unknown. Using new methods, we determined P in DNA and phospholipids, which are both found in all organisms. We also identified inositol phosphates that are particularly important in eukaryotes. Sediment cores were collected from contrasting basins in the Baltic Sea to study their relative contribution to the total P pool. We found high DNA-P/phospholipid-P ratios in surface sediments from the Bothnian Bay and Bothnian Sea. However, these ratios were low throughout profiles in euxinic Baltic Proper sediments. The elevated ratios present in sediments overlain by oxic bottom waters might indicate the presence of a microbial community stimulated by bioturbation, whereas the low DNA-P/phospholipid-P ratios in Baltic Proper sediments likely indicate an energy-limited microbial community, typical to the “deep biosphere” environment. Inositol-P was almost absent in euxinic Baltic Proper sediments that had a low total P amount compared to those in the other basins. We suggest that variability in the composition of sedimentary microbial communities among the Baltic Sea basins might cause differences in organic P forms that in turn affects its turnover. 

Sedimentary molybdenum (Mo) and uranium (U) enrichments are often used as redox proxies to reconstruct bottom water redox changes. However, these redox proxies may not be equally reliable across a range of coastal settings due to varying depositional environments. Fjords vary greatly in their depositional conditions, due to their unique bathymetry and hydrography, and are highly vulnerable to anthropogenic and climatic pressures. Currently, it is unknown to what extent Mo and U sequestration is affected by variable depositional conditions in fjords. Here, we use pore water and sequential extraction data to investigate Mo and U enrichment pathways in sediments of two sill fjords on the Swedish west coast with contrasting depositional environments and bottom water redox conditions. Our data suggest that sedimentary authigenic Mo and U pools differ between the two fjords. At the (ir)regularly dysoxic (oxygen = 0.2–2 mL L⁻¹) Gullmar Fjord, authigenic Mo largely binds to manganese (Mn) oxides and to a lesser extent to iron (Fe) oxides; Mo sulfides do not play a major role due to low sulfate reduction rates, which limits the rate of Mo burial. Authigenic U largely resides in carbonates. At the (ir)regularly euxinic (oxygen = 0 mL L⁻¹; total hydrogen sulfide ≥ 0 mL L⁻¹) Koljö Fjord, authigenic Mo is significantly higher due to binding with more refractory organic matter complexes and Mo-Fe-sulfide phases. Uranium is moderately enriched and largely bound to organic matter. We found no direct evidence for temporal changes in bottom water redox conditions reflected in Mo and U enrichments at either Gullmar Fjord or Koljö Fjord. While sulfidic bottom waters favor Mo sequestration at Koljö Fjord, enrichment maxima reflect a combination of depositional conditions rather than short-term low-oxygen events. Our data demonstrate that secondary pre- and post-depositional factors control Mo and U sequestration in fjords to such an extent that bottom water redox conditions are either not being systematically recorded or overprinted. This explains the large variability in trace metal enrichments observed in fjords and has implications for applying Mo and U as proxies for environmental redox reconstructions in such systems.

Hypoxia has occurred intermittently in the Baltic Sea since the establishment of brackish-water conditions at ∼8,000 years B.P., principally as recurrent hypoxic events during the Holocene Thermal Maximum (HTM) and the Medieval Climate Anomaly (MCA). Sedimentary phosphorus release has been implicated as a key driver of these events, but previous paleoenvironmental reconstructions have lacked the sampling resolution to investigate feedbacks in past iron-phosphorus cycling on short timescales. Here we employ Laser Ablation (LA)-ICP-MS scanning of sediment cores to generate ultra-high resolution geochemical records of past hypoxic events. We show that in-phase multidecadal oscillations in hypoxia intensity and iron-phosphorus cycling occurred throughout these events. Using a box model, we demonstrate that such oscillations were likely driven by instabilities in the dynamics of iron-phosphorus cycling under preindustrial phosphorus loads, and modulated by external climate forcing. Oscillatory behavior could complicate the recovery from hypoxia during future trajectories of external loading reductions.