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Project

Project type/Form of grant
Grant for employment or scholarship
Title [sv]
Funktionell och strukturell karaktärisering av gram-positiva typ 4 sekretionssystem
Title [en]
Structural and functional characterisation of Gram-positive Type 4 Secretion Systems
Abstract [en]
Multiresistant bacteria are an ever-growing threat in today?s society. Genes encoding for antibiotic resistance are spread between bacteria in horizontal gene transfer events, often via Type 4 Secretion Systems (T4SS). The goal of this project is to determine the molecular structure and function of T4SSs from gram-positive (G+) bacteria, which have not been well studied and are very different from their gram-negative (G-) counterparts.The specific aims of this project are to (i) determine the structures of the energetic and adhesion proteins from the T4SS originating from the pCF10 plasmid in Enteroccus faecalis (years 1-3), (ii) functionally characterize the energetic and adhesion components by in vivo conjugation assays and in vitro binding studies using ITC and SPR (years 2-4) and (iii) determine the structure of the core T4SS channel of the same system in situ, using Cryo-electron Tomography (years 2-4+).The significance of this research is twofold. First, it will provide answers to a number of fundamental scientific questions regarding the basic function and structure of G+ T4SSs. Second, it will provide a foundation for future drug development against these types of pathogens, which currently are highly prevalent in hospital infections. It is a highly relevant project that will fill large black gaps in our current knowledge, and its importance only grows when viewed in the light of the increasing prevalence of multiresistant bacteria in today?s society.
Principal InvestigatorBerntsson, Ronnie
Coordinating organisation
Umeå University
Funder
Period
2017-01-01 － 2020-12-31
National Category
Structural BiologyBiochemistry and Molecular BiologyBiophysics
Identifiers
DiVA, id: project:1431Project, id: 2016-03599_VR

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