The naturally occurring isotopes ²³¹Pa and ²³⁰Th are used as tracers of marine biogeochemical processes. They are both produced from the radioactive decay of their uniformly distributed uranium parents (²³⁵U and ²³⁴U) in seawater. After production, ²³¹Pa and ²³⁰Th are removed by adsorption onto settling particles (scavenging) and subsequently buried in marine sediments. ²³⁰Th is more particle reactive compared to ²³¹Pa. Consequently, ²³⁰Th will be removed from the open ocean by adsorption onto settling particles, while ²³¹Pa tend to be laterally transported by currents and removed by scavenging in areas of high particle flux (e.g. ocean margins). The primordial ²³²Th indicates lithogenic supply via rivers and resuspension of sediments, which provides additional information about processes involved in the cycling of particle reactive elements in the ocean. The preferential deposition of particle reactive elements at ocean margins (boundary scavenging) has important implications for our understanding of the distribution and dispersion of micronutrients (e.g. iron) and pollutants in the ocean. It is therefore valuable to understand the nature of boundary scavenging processes in order to evaluate the relative contribution of circulation and scavenging behaviors.The major characteristics of thermohaline circulation in the Mediterranean are well known and have been studied for decades. This sea is an almost land-locked area, where limited water-exchange with the Atlantic Ocean only occurs through the Strait of Gibraltar. Therefore, this marginal sea is often referred to as a “miniature ocean” suitable as a “laboratory” for marine environmental research. In this licentiate thesis, distributions of ²³¹Pa, ²³⁰Th and ²³²Th in seawater and marine particles collected during the GEOTRACES MedSeA-GA04-S cruise in 2013 are presented. Observed nuclide distributions indicate the impact of deep water formation processes, where observed differences can be linked to the type of deep water formation process that occurs in respective basin. Essentially all in-situ produced ²³⁰Th is buried in Mediterranean Sea sediments. Despite lower affinity of ²³¹Pa for marine particles, most ²³¹Pa is also scavenged and deposited in Mediterranean Sea sediments. The efficient scavenging of ²³¹Pa produces a relatively low fractionation between ²³¹Pa and ²³⁰Th in terms of the fractionation factor F_Th/Pa. This licentiate thesis presents a summary of the methods used for the analysis of ²³¹Pa and Th-isotopes with details on the exchange chromatography method and the treatment of mass spectrometric data. The study of ²³¹Pa, ²³⁰Th and ²³²Th in the Mediterranean Sea has important implications for our understanding of processes that control their water column distributions and how their behavior can be utilized to trace chemical flux in modern and past ocean environments.