Using diatoms, pollen, and geochemistry, we explore human habitation around Lina myr, Gotland, in relation to shore displacement. Archeological evidence has shown that Lina myr was an important area for its prehistoric human inhabitants. We investigate if and when Lina myr was connected to the sea and could therefore have been part of an inland water system useful for transport. A chronology was based on C-14 AMS dating of terrestrial macrofossils and bulk sediments with dates ranging between 9100 and 2360 cal. yr BP. The initiation of the Littorina transgression was dated to 8500 cal. yr BP. A twofold pattern for the maximum sub-phase of the Littorina Sea is suggested from 8100 to 7500 cal. yr BP and from 6500 to 6000 cal. yr BP. The onset of cultivation and grazing was indicated by the presence of Hordeum and Plantago lanceolata in the pollen record during the Late Neolithic, at about 4580 cal. yr BP. During this time sea level was relatively higher than today and the Lina myr basin was connected with the Littorina Sea, which it continued to be until isostatic uplift caused it to become isolated at about 3820 cal. yr BP. After about 3000 cal. yr BP, human-made landscape changes intensified, grasslands increased, and shrublands decreased.