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Altered gut microbiota community structure and correlated immune system changes in dibutyl phthalate exposed mice
Stockholm University, Faculty of Science, Department of Environmental Science. Stockholm University, Science for Life Laboratory (SciLifeLab).
Stockholm University, Science for Life Laboratory (SciLifeLab). Stockholm University, Faculty of Science, Department of Environmental Science.ORCID iD: 0000-0002-6111-7435
Stockholm University, Science for Life Laboratory (SciLifeLab). Stockholm University, Faculty of Science, Department of Environmental Science.
Number of Authors: 42023 (English)In: Ecotoxicology and Environmental Safety, ISSN 0147-6513, E-ISSN 1090-2414, Vol. 262, article id 115321Article in journal (Refereed) Published
Abstract [en]

Di-n-butyl phthalate (DBP) is a ubiquitous environmental contaminant linked with various adverse health effects, including immune system dysfunction. Gut microbial dysbiosis can contribute to a wide range of pathogenesis, particularly immune disease. Here, we investigated the impact of DBP on the gut microbiome and examined correlations with immune system changes after five weeks oral exposure (10 or 100 mg/kg/day) in adult male mice. The fecal microbiome composition was characterized using 16S rRNA sequencing. DBP-treated mice displayed a significantly distinct microbial community composition, indicated by Bray-Curtis distance. Numerous amplicon sequence variants (ASVs) at the genus level were altered. Compared to the vehicle control group, the 10 mg/kg/day DBP group had 63 more abundant and 65 less abundant ASVs, while 60 ASVs were increased and 76 ASVs were decreased in the 100 mg/kg/day DBP group. Both DBP treatment groups showed higher abundances of ASVs assigned to Desulfovibrio (Proteobacteria phylum) and Enterorhabdus genera, while ASVs belonging to Parabacteroides, Lachnospiraceae UCG-006 and Lachnoclostridium were less common compared to the control group. Interestingly, an ASV belonging to Rumniniclostridium 6, which was less abundant in DBP-treated mice, demonstrated a negative correlation with the increased number of non-classical monocytes observed in the blood of DBP-treated animals. In addition, an ASV from Lachnospiraceae UCG-001, which was more abundant in the DBP-treated animals, showed a positive correlation with the non-classical monocyte increase. This study shows that DBP exposure greatly modifies the gut bacterial microbiome and indicates a potential contribution of microbial dysbiosis to DBP-induced immune system impairment, illustrating the importance of investigating how interactions between exposome components can affect health.

Place, publisher, year, edition, pages
2023. Vol. 262, article id 115321
Keywords [en]
DBP, Gut microbiota, Phthalates, Endocrine disruptor, EDC, Environmental contaminant, Exposome
National Category
Earth and Related Environmental Sciences Basic Medicine
Identifiers
URN: urn:nbn:se:su:diva-220848DOI: 10.1016/j.ecoenv.2023.115321ISI: 001054919500001PubMedID: 37549549Scopus ID: 2-s2.0-85166521023OAI: oai:DiVA.org:su-220848DiVA, id: diva2:1796726
Available from: 2023-09-13 Created: 2023-09-13 Last updated: 2024-01-24Bibliographically approved
In thesis
1. Toxicological studies of di-n-butyl phthalate (DBP): Impact on the reproductive system and gut microbiota
Open this publication in new window or tab >>Toxicological studies of di-n-butyl phthalate (DBP): Impact on the reproductive system and gut microbiota
2024 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The potential health impact of exposure to anthropogenic chemicals has raised major concerns worldwide. Phthalates are mainly used in the plastic industry and have been associated with adverse effects in humans. Di-n-butyl phthalate (DBP) is one of the dominant phthalates with a ubiquitous presence in the environment. While many studies have examined the endocrine disrupting properties of DBP, with a focus on developmental and reproductive dysfunctions, studies of its effects on the adult reproductive system and gut microbiota are limited. This thesis aimed to determine persistent effects of DBP on the adult male reproductive system, provide a high-throughput screening tool for identifying reproductive toxicants, and characterize the effects of DBP on the gut microbiota.

   Paper I investigated if adult DBP exposure can induce persistent effects on the mature reproductive system. Adult male mice were orally exposed to 10 or 100 mg/kg/day for five weeks and testes were collected one week after the last dose. The results demonstrated a significant decrease in testosterone levels in the DBP-exposed groups. Mechanistically, the levels of steroidogenic enzymes, cell-specific markers and oxidative stress were increased. In paper II, elements of the in vivo testicular microenvironment, including functional testosterone production, were modeled using a three-dimensional (3D) heterogenous testicular cell co-culture derived from neonatal mice. Automated high-content imaging of cell-specific markers confirmed the presence of germ cells (DAZL+), Leydig cells (CYP11A1+), and Sertoli cells (SOX9+). DBP exposure decreased testosterone production, as well as levels of the steroidogenic enzyme CYP11A1, and the steroidogenic regulator StAR. Overall, this in vitro 3D model recapitulates the testicular pathways involved in DBP toxicity, making it a relevant tool for assessing reprotoxic effects of chemicals.

   Paper III investigated the impact of oral DBP exposure on the gut microbiota and the potential interplay with immune and testicular toxicity using 16S rRNA sequencing. DBP-treated mice showed a distinct microbial composition and numerous differentially abundant amplicon sequence variants. Interestingly, the microbial alterations correlated with an increase in non-classical monocytes observed in DBP-exposed mice. In paper IV, a shotgun metagenomic analysis was conducted to achieve a more comprehensive characterization of the DBP-induced effects on gut microbiota composition and function. The DBP-exposed mice had a higher abundance of Adlercreutzia mucosicola, a bacterium linked with intestinal inflammation. In contrast, the beneficial Akkermansia muciniphila was less abundant in DBP-exposed mice. Functional analysis demonstrated that DBP exposure increased the abundance of genes involved in environmental sensing and antimicrobial resistance.

   In conclusion, this doctoral thesis demonstrates the antiandrogenic effects of DBP as well as potential underlying mechanisms of testicular dysfunction in adult mice. In addition, we established a powerful in vitro tool for screening reprotoxic effects. The gut microbiota was also impaired by DBP exposure, which may play a potential role in initiating or exacerbating the DBP-induced toxicity. Overall, this work highlights the potential health impact of the interplay between the two exposome components, chemical exposure and gut microbiota.

Place, publisher, year, edition, pages
Stockholm: Department of Environmental Science, Stockholm University, 2024. p. 56
Keywords
Dibutyl phthalate, Antiandrogen, Gut microbiota, Reproductive toxicity, Endocrine disrupting chemicals, Environmental contaminants, Phthalates, Plastics, 3D cell culture model, Exposome, Toxicology
National Category
Pharmacology and Toxicology
Research subject
Environmental Sciences
Identifiers
urn:nbn:se:su:diva-225853 (URN)978-91-8014-651-7 (ISBN)978-91-8014-652-4 (ISBN)
Public defence
2024-03-08, De Geersalen, Geovetenskapens hus, Svante Arrhenius väg 14, Stockholm, 09:00 (English)
Opponent
Supervisors
Available from: 2024-02-14 Created: 2024-01-24 Last updated: 2024-02-08Bibliographically approved

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Almamoun, RadwaPierozan, PaulaKarlsson, Oskar

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