The postglacial recolonization of Fennoscandian flora and fauna was initiated when the land became accessible as the last ice sheet retreated. In northern Sweden, plants are represented in pollen and macrofossil records, but there is no genetic evidence from the first plants, animals or humans in the region, mainly owing to an absence of osteological finds. The questions of who the first postglacial peoples, or pioneers, were and where they came from therefore remain unanswered. Previous palaeogenomic analyses from remains from adjacent regions have suggested that two main routes into Sweden could have been taken by the pioneers, one from the SW through modern-day Denmark and Norway, and one from the east via Finland. However, no direct genetic evidence from the pioneers of northern Sweden exists. Modern technology has provided the ancient DNA field with an updated toolbox that could allow for novel approaches for revealing the origin and genetic profiles of the first Scandinavians, of which sedimentary ancient DNA (sedaDNA) is well placed. Lake sediments are now a routine source of sedaDNA that have been used to record environmental changes and detect species that lived in the surrounding lake catchment. This review will provide context and background, a summary of the ground-breaking studies within the field of lacustrine sedaDNA, and relevant methodology to address the scientific questions at hand. We conclude that the field is mature enough to provide insight into the origins and arrival times of the first postglacial humans that migrated into northern Sweden.