This paper proposes an empirical research agenda for investigating the practices of biosecuritization of wild animal threats in modern society. Previously mostly studied on the lofty biopolitical level of directives on combatting invasive species or culling pests, we outline the conceptual and methodological points of entry for bringing the on-the-ground work of culling out-of-place, unwanted, individual animals and populations. This means a focus on necropolitics as constituted by the norms, everyday professional practices, vernaculars of killing, and identity work by pest controllers in the city and hunters on the countryside. Borrowing from research in the domestic animal killing context, we nevertheless show how wild animal killing is imbued with more spontaneity, remorse, aesthetics, public stigma, and multispecies entanglements, requiring adapted research protocols.