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Biophysics of SARS-CoV-2 spike protein’s receptor-binding domain interaction with ACE2 and neutralizing antibodies: from computation to functional insights
Stockholm University, Faculty of Science, Department of Chemistry. Nanjing Tech University, People’s Republic of China; Luleå University of Technology, Sweden; Petru Poni Institute of Macromolecular Chemistry, Romania.ORCID iD: 0000-0001-9783-4535
Number of Authors: 42025 (English)In: Biophysical Reviews, E-ISSN 1867-2469, Vol. 17, no 2, p. 309-333Article, review/survey (Refereed) Published
Abstract [en]

The spike protein encoded by the SARS-CoV-2 has become one of the most studied macromolecules in recent years due to its central role in COVID-19 pathogenesis. The spike protein’s receptor-binding domain (RBD) directly interacts with the host-encoded receptor protein, ACE2. This review critically examines computational insights into RBD’s interaction with ACE2 and with therapeutic antibodies designed to interfere with this interaction. We begin by summarizing insights from early computational studies on pre-pandemic SARS-CoV-1 RBD interactions and how these early studies shaped the understanding of SARS-CoV-2. Next, we highlight key theoretical contributions that revealed the molecular mechanisms behind the binding affinity of SARS-CoV-2 RBD against ACE2, and the structural changes that have enhanced the infectivity of emerging variants. Special attention is given to the “RBD charge rule”, a predictive framework for determining variant infectivity based on the electrostatic properties of the RBD. Towards applying the computational insights to therapy, we discuss a multiscale computational protocol for optimizing monoclonal antibodies to improve binding affinity across multiple spike protein variants, including representatives from the Omicron family. Finally, we explore how these insights can inform the development of future vaccines and therapeutic interventions for combating future coronavirus diseases.

Place, publisher, year, edition, pages
2025. Vol. 17, no 2, p. 309-333
Keywords [en]
Antibodies, Complexation, Electrostatic interactions, Molecular simulation, pH effects, Transmissibility, Virus
National Category
Biophysics
Identifiers
URN: urn:nbn:se:su:diva-243035DOI: 10.1007/s12551-025-01276-zISI: 001439501200001Scopus ID: 2-s2.0-86000621168OAI: oai:DiVA.org:su-243035DiVA, id: diva2:1957089
Available from: 2025-05-08 Created: 2025-05-08 Last updated: 2025-10-28Bibliographically approved

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Laaksonen, Aatto

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