Background: Harmful alcohol consumption has significant negative implications for the workplace. The workplace offersa strategic opportunity for alcohol prevention due to the substantial time employees spend at work.

Objective: Utilizing a social-ecological framework, this study aims to investigate whether universal alcohol preventionstrategies in the workplace are associated with employees’ alcohol-related perceptions or behaviors

Methods: A cross-sectional survey study was conducted in December 2019 using a web-based questionnaire distributedthrough the Laboratory of Opinion Research Citizen Panel. Participants included 2771 employed adults aged 16–80 inSweden. Multiple- and logistic regressions were used to investigate significant explanatory factors.

Results: Having alcohol procedures, as well as having received information from a supervisor regarding how to act onconcerns, were associated with a higher probability of being comfortable informing a supervisor of concerns. A restrictivealcohol culture was associated with lower threshold for perceived risk-free alcohol consumption. Having an alcohol policyonly was not associated with any of the examined alcohol-related perceptions or behaviors when controlling for otherfactors.

Conclusions: Alcohol preventive measures can significantly influence employee perceptions of responsibility to act onconcerns, and the feeling of being comfortable doing so. The findings support the integration of alcohol preventive measuresat multiple levels within the workplace, as well as having a restrictive alcohol culture.