Digital transformation (DT) has significantly reshaped many organizations’ business models, processes, and strategies, focusing on creating value propositions for stakeholders. Despite its potential, DT often encounters challenges rooted in organizational culture. However, research on these cultural barriers remains fragmented, leaving a knowledge gap in understanding how they hinder transformation. This study systematically reviews existing research literature to identify and analyze the organizational culture barriers that impede DT. The systematic review reveals 10 key and 44 specific organizational cultural barriers to successful DT. To address them, the study proposes targeted solutions framed within elements of adaptive culture, recognizing that these cultural barriers can hinder the development of adaptive culture that supports successful DT. This proposed approach contributes a structured framework that synthesizes existing findings and offers insights to guide organizational practice, academic research, and policymaking related to DT.