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Molecular phylogenetics reveals novel relationships within Empidoidea (Diptera)
Stockholm University, Faculty of Science, Department of Zoology. Swedish Museum of Natural History, Sweden.
2018 (English)In: Systematic Entomology, ISSN 0307-6970, E-ISSN 1365-3113, Vol. 43, no 4, p. 619-636Article in journal (Refereed) Published
Abstract [en]

Empidoidea represent a large and diverse superfamily of true flies, and to date no stable hypothesis on the phylogeny exists. Previous classifications have been based on morphological data and the relationships among several groups are still unknown. Using the mitochondrial genes cytochrome oxidase c subunit I (COI) and cytochrome β (Cytβ) and the nuclear genes carbomoylphosphate synthase domain of rudimentary (CAD), elongation factor‐1α (EF‐1α) and isocitrate dehydrogenase (IDH) in a Bayesian analysis, we tested the support of higher taxonomic groups within this large superfamily of flies. We re‐evaluated previous hypotheses of evolution within the group and present a highly supported phylogenetic hypothesis. Atelestidae, Dolichopodidae, Empididae and Hybotidae were supported as monophyletic families, with Atelestidae as sister group to the remaining Empidoidea. Within the family Hybotidae, Bicellariinae stat.n. formed the sister group to the other subfamilies. The family Ragadidae stat.n. is established to include the subfamily Ragadinae and the new subfamily Iteaphilinae subfam.n.; Ragadidae was sister group to the Empididae. Dolichopodidae was found to form a sister group to Ragadidae plus Empididae. Within Empididae, Hemerodromiinae was found to be a nonmonophyletic group. The tribes Hilarini and Hemerodromiini stat. rev. were recovered as sister groups, as were Empidini and Chelipodini stat. rev. The former family Brachystomatidae was found to be nested within Empididae. A revised classification and diagnoses of nondolichopodid families, subfamilies and tribes are provided.

Place, publisher, year, edition, pages
2018. Vol. 43, no 4, p. 619-636
National Category
Biological Systematics
Research subject
Systematic Zoology
Identifiers
URN: urn:nbn:se:su:diva-154900DOI: 10.1111/syen.12297ISI: 000443411300001OAI: oai:DiVA.org:su-154900DiVA, id: diva2:1195722
Funder
The Swedish Taxonomy Initiative (ArtDatabanken, SLU), dha 2014‐149 4.3Available from: 2018-04-06 Created: 2018-04-06 Last updated: 2022-02-26Bibliographically approved
In thesis
1. Molecules and morphology in Empidoidea (Diptera): Phylogenetic analysis and taxonomical implications
Open this publication in new window or tab >>Molecules and morphology in Empidoidea (Diptera): Phylogenetic analysis and taxonomical implications
2020 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The classification and evolutionary history of the superfamily Empidoidea (Diptera) have been far from straightforward subjects. Morphology based phylogenies of the superfamily have been unable to answer many questions raised by both systematists and biogeographers, as well as ecologists and ethologists, partly due to morphological modifications and adaptations difficult to interpret. In paper I a phylogeny is presented which was the first published that was based on molecular data with multiple loci and with high support. We sampled several groups never included in molecular analyses before and were able to resolve several relationships and recover several species groups that were previously unplaced. Atelestidae is confirmed as the sister group to remaining families. Hybotidae is sister group to Dolichopodidae, Ragadidae stat. n. and Empididae. Species included in the family Brachystomatidae are found to be nested within Empididae and Brachystomatidae is therefore lowered to subfamily. The erection of Ragadidae to family, sister to Empididae, is based on genetic distances between the present families and subfamilies. Paper II deals with the genus Wiedemannia (Empididae: Clinocerinae), also using molecular data but also put into a spatial and temporal context with molecular dating. The subgenera are found to be non-monophyletic and are therefore suggested to be rejected as accepted names. The dated tree, based on molecular data and known fossil records, suggests an initial diversification 50 million years ago followed by further diversification events that may be linked to changes in sea levels on a global scale. We also provide a Species Distribution Model map suggesting areas of potential hot spots as based on climatic variables. Following the results in paper I, the internal relationships of Ragadidae are revised in paper III. The lack of DNA data, partly caused by few available specimens and the rarity of some species, motivated a morphological approach together with a maximum parsimony analysis to investigate the monophyly of species groups and genera. The flower feeding genera Iteaphila and Anthepiscopus are found to form a distinct monophyletic clade together with Hormopeza. The internal relationships are revised, updated diagnostic characters are provided as well as a determination key to the genera. In paper IV we deal with the taxonomical issue of Chvalaea sopianae and C. rugosiventris, two species in the family Hybotidae. Both species were originally described from single female specimens.  However, the sex of C. rugosiventris was mistaken in the original description, and the differential characters of the two species are based on sexual dimorphism. The two species are synonymized and the male of C. rugosiventris redescribed. We also provide additional geographic data on the hybotid species Allanthalia pallida and Leptodromiella crassiseta. The final paper, paper V, presents records of new species to Sweden and new records within Sweden of a large number of species of Hybotidae, Empididae and Ragadidae. We also provide a substantial addition of genetic barcodes of the Swedish fauna, shared publicly in The Barcode of Life Database. This increases the coverage of Swedish taxa in the database with 71% for Empididae and 13% for Hybotidae.

Place, publisher, year, edition, pages
Stockholm: Department of Zoology, Stockholm University, 2020. p. 36
Keywords
Empidoidea, Empididae, Hybotidae, phylogeny, taxonomy, systematics, molecular data, morphology, revision
National Category
Zoology
Research subject
Systematic Zoology
Identifiers
urn:nbn:se:su:diva-179033 (URN)978-91-7911-070-3 (ISBN)978-91-7911-071-0 (ISBN)
Public defence
2020-04-24, Lilla hörsalen, Naturhistoriska riksmuséet, Frescativägen 40, Stockholm, 13:00 (English)
Opponent
Supervisors
Funder
The Swedish Taxonomy Initiative (ArtDatabanken, SLU), dha 2014-149 4.3
Available from: 2020-04-01 Created: 2020-02-19 Last updated: 2022-02-26Bibliographically approved

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