Renewable resources such as wood are an important candidate towards the replacement of fossil-based materials with bio-based materials. Lignin, comprising up to 40% of woody biomass, has shown great potential in the preparation of nanoparticles (LNPs), which can be used in diverse material applications. Typically, LNPs are prepared from technical lignins, deriving from pulping processes. Their formation is thought to be driven by the hydroxyl content and molecular weight of lignin. However, other equally important parameters are the monolignol composition and the concentration of lignin used. In this study, we used native-like lignin fractions from hardwood and softwood to investigate the impact of lignin structure on LNP formation. We identified a synergistic effect between 7C-7C stacking and hydroxyl group content in nanoparticle formation. Our LNPs exhibited different morphologies, including compact, collapsed spheres, and 'snowman' aggregates. The results described herein provide an in-depth perspective on the formation and structure-property relationship of LNPs from native -like lignin fractions. With this study we aim to promote biorefinery concepts, in which the lignin structure is preserved during extraction.